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Literature summary for 6.1.1.14 extracted from

  • Wu, H.; Nada, S.; Dignam, D.
    Analysis of truncated forms of Bombyx mori glycyl-tRNA synthetase: Function of an N-terminal structure in RNA binding (1995), Biochemistry, 34, 16327-16336.
    View publication on PubMed
Search for more literature for 6.1.1.14

Protein Variants

Protein Variants Comment Organism
additional information truncated enzyme forms with deletions of 12, 27, 46, and 55 N-terminal residues reduce the kcat value of the wild-type enzyme by a factor 5-10 in diphosphate exchange and aminoacylation activity, but does not significantly change the Km of the three substrates. Deletions of 108 N-terminal residues or the internal segments 111-164 and 110-309 cause complete loss of activity. Deletions from the C-terminus of 24, 38, 60, 163, and 328 residues result in inactive enzyme forms. Whereas the wild-type enzyme binds both tRNAGly and noncognate tRNAAla, the mutant lacking 55 N-terminal residues shows altered binding of tRNAGly and does not bind tRNAAla Bombyx mori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00035
-
 Gly wild-type enzyme Bombyx mori
0.00055
-
 Gly N55-truncation mutant Bombyx mori
0.001
-
 tRNAGly N55-truncation mutant enzyme Bombyx mori
0.0012
-
 tRNAGly wild-type enzyme Bombyx mori
0.013
-
 ATP wild-type enzyme Bombyx mori
0.014
-
 ATP N55-truncation mutant enzyme Bombyx mori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76919
-
 x * 76919, wild-type enzyme, calculation from nucleotide sequence Bombyx mori
155000
-
 gel filtration, calculation from sedimentation constant Bombyx mori

Organism

Organism UniProt Comment Textmining
Bombyx mori
-
 wild-type and truncated enzyme forms
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + glycine + tRNAGly the wild-type enzyme binds both tRNAGly and noncognate tRNAAla. The mutant lacking 55 N-terminal residues shows altered binding of tRNAGly and does not bind tRNAAla Bombyx mori AMP + diphosphate + glycyl-tRNAGly
-
 ?

Subunits

Subunits Comment Organism
? x * 76919, wild-type enzyme, calculation from nucleotide sequence Bombyx mori