BRENDA - Enzyme Database
show all sequences of 6.1.1.13

D-Alanine:membrane acceptor ligase from Lactobacillus casei

Reusch, V.M.; Neuhaus, F.C.; J. Biol. Chem. 246, 6136-6143 (1971)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Lactobacillus casei
D-2-Amino-n-butyric acid hydroxamate
-
Lactobacillus casei
D-Ser
-
Lactobacillus casei
DL-2-Amino-n-butyric acid hydroxamate
-
Lactobacillus casei
DL-Alanine amide
-
Lactobacillus casei
DL-Alanine hydroxamate
-
Lactobacillus casei
KCl
-
Lactobacillus casei
additional information
not inhibitory are D-Ala, L-Ala, L-2-amino-n-butyric acid, Gly, D-Thr, D-Ile, L-Ile, D-cycloserine, L-cycloserine, DL-alaniol
Lactobacillus casei
NaCl
-
Lactobacillus casei
Ni2+
-
Lactobacillus casei
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
D-Ala
-
Lactobacillus casei
0.85
-
D-2-amino-n-butyric acid
-
Lactobacillus casei
3.3
-
ATP
-
Lactobacillus casei
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Lactobacillus casei
16020
-
soluble
-
Lactobacillus casei
-
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
Ba2+ can replace Mg2+ in activity enhancement
Lactobacillus casei
Ca2+
Ca2+ can replace Mg2+ in activity enhancement
Lactobacillus casei
Co2+
Co2+ can replace Mg2+ in activity enhancement
Lactobacillus casei
K+
K+ can replace Mg2+ in activity enhancement
Lactobacillus casei
Mg2+
enhancement of activity, Mg2+ can be replaced by Ba2+, Ca2+, Co2+, K+, Na+
Lactobacillus casei
Na+
Na+ can replace Mg2+ in activity enhancement
Lactobacillus casei
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
gel filtration
Lactobacillus casei
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-Alanine + ATP + ?
Lactobacillus casei
biosynthesis of teichoic acid
?
-
-
-
Organism
Organism
UniProt
Commentary
Textmining
Lactobacillus casei
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partial
Lactobacillus casei
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
ATP + D-2-amino-n-butyric acid + membrane fragments
not L-2-amino-n-butyric acid
1056
Lactobacillus casei
D-2-Amino-n-butryl-membrane fragments + AMP + diphosphate
-
1056
Lactobacillus casei
-
ATP + D-Ala + membrane fragments
ATP can be substituted by ADP, GTP, CTP, UTP, not by AMP, D-Ala cannot be substituted by L-Ala
1056
Lactobacillus casei
D-Alanyl-membrane fragments + AMP + diphosphate
-
1056
Lactobacillus casei
-
D-Alanine + ATP + ?
biosynthesis of teichoic acid
1056
Lactobacillus casei
?
-
-
-
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Lactobacillus casei
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
-
Lactobacillus casei
D-2-Amino-n-butyric acid hydroxamate
-
Lactobacillus casei
D-Ser
-
Lactobacillus casei
DL-2-Amino-n-butyric acid hydroxamate
-
Lactobacillus casei
DL-Alanine amide
-
Lactobacillus casei
DL-Alanine hydroxamate
-
Lactobacillus casei
KCl
-
Lactobacillus casei
additional information
not inhibitory are D-Ala, L-Ala, L-2-amino-n-butyric acid, Gly, D-Thr, D-Ile, L-Ile, D-cycloserine, L-cycloserine, DL-alaniol
Lactobacillus casei
NaCl
-
Lactobacillus casei
Ni2+
-
Lactobacillus casei
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.018
-
D-Ala
-
Lactobacillus casei
0.85
-
D-2-amino-n-butyric acid
-
Lactobacillus casei
3.3
-
ATP
-
Lactobacillus casei
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
membrane
-
Lactobacillus casei
16020
-
soluble
-
Lactobacillus casei
-
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
Ba2+ can replace Mg2+ in activity enhancement
Lactobacillus casei
Ca2+
Ca2+ can replace Mg2+ in activity enhancement
Lactobacillus casei
Co2+
Co2+ can replace Mg2+ in activity enhancement
Lactobacillus casei
K+
K+ can replace Mg2+ in activity enhancement
Lactobacillus casei
Mg2+
enhancement of activity, Mg2+ can be replaced by Ba2+, Ca2+, Co2+, K+, Na+
Lactobacillus casei
Na+
Na+ can replace Mg2+ in activity enhancement
Lactobacillus casei
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
39000
-
gel filtration
Lactobacillus casei
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-Alanine + ATP + ?
Lactobacillus casei
biosynthesis of teichoic acid
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Lactobacillus casei
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
ATP + D-2-amino-n-butyric acid + membrane fragments
not L-2-amino-n-butyric acid
1056
Lactobacillus casei
D-2-Amino-n-butryl-membrane fragments + AMP + diphosphate
-
1056
Lactobacillus casei
-
ATP + D-Ala + membrane fragments
ATP can be substituted by ADP, GTP, CTP, UTP, not by AMP, D-Ala cannot be substituted by L-Ala
1056
Lactobacillus casei
D-Alanyl-membrane fragments + AMP + diphosphate
-
1056
Lactobacillus casei
-
D-Alanine + ATP + ?
biosynthesis of teichoic acid
1056
Lactobacillus casei
?
-
-
-
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7
-
Lactobacillus casei
Other publictions for EC 6.1.1.13
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
726624
Huang
Expression, purification, crys ...
Staphylococcus epidermidis
Acta Crystallogr. Sect. F
68
810-812
2012
-
-
1
1
-
-
-
-
-
-
-
1
-
3
-
-
1
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
727754
Guariglia-Oropeza
Bacillus subtilis delta(V) con ...
Bacillus subtilis 168, Bacillus subtilis
J. Bacteriol.
193
6223-6232
2011
-
-
-
-
-
-
-
-
-
-
-
2
-
92
-
-
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651678
Kiriukhin
D-alanylation of lipoteichoic ...
Lactobacillus casei 102S, Lactobacillus casei
J. Bacteriol.
183
2051-2058
2001
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
651652
Debabov
Biosynthesis of lipoteichoic a ...
Lactobacillus rhamnosus, Lactobacillus rhamnosus 7469
J. Bacteriol.
182
2855-2864
2000
1
-
-
-
-
-
-
-
-
-
-
2
-
6
-
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210879
Debabov
The D-alanyl carrier protein i ...
Lactobacillus casei
J. Bacteriol.
178
3869-3876
1996
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
210877
Perego
Incorporation of D-alanine int ...
Bacillus subtilis
J. Biol. Chem.
270
15598-15606
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1056
Reusch
D-Alanine:membrane acceptor li ...
Lactobacillus casei
J. Biol. Chem.
246
6136-6143
1971
-
-
-
-
-
-
10
3
3
6
1
1
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
10
-
3
3
6
1
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
210876
Baddiley
The enzymic activation of D-al ...
Lactobacillus plantarum
Biochem. J.
75
579-587
1960
-
-
-
-
-
-
-
4
-
-
-
-
-
1
-
-
1
-
-
-
1
2
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
1
-
-
1
2
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-