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Literature summary for 6.1.1.12 extracted from
- Dual targeting of a tRNAAsp requires two different aspartyl-tRNA synthetases in Trypanosoma brucei (2009), J. Biol. Chem., 284, 16210-16217.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the sequence corresponding to the carboxyl-terminal Ty1-tagged Tb-AspRS1 is cloned into a derivative of pLew-100 to allow tetracycline-inducible expression of the tagged protein in transgenic cell lines | Trypanosoma brucei |
| the sequence corresponding to the carboxyl-terminal Ty1-tagged Tb-AspRS1 is cloned into a derivative of pLew-100 to allow tetracycline-inducible expression of the tagged protein in transgenic cell lines, into the vector pET15b for expression in Escherichia coli BL21-CodonPlus DE3-RIL cells | Trypanosoma brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| RNAi | - |
Trypanosoma brucei |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Trypanosoma brucei | 5829 | - |
| mitochondrion | - |
Trypanosoma brucei | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60800 | - |
theoretical | Trypanosoma brucei |
| 62900 | - |
theoretical | Trypanosoma brucei |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate + tRNAAsp | Trypanosoma brucei | - |
AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | Q38CB4 | - |
- |
| Trypanosoma brucei | Q585M3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by Ni2+-nitrilotriacetic acid chromatography, cytosolic and mitochondrial fractions of Trypanosoma brucei are prepared | Trypanosoma brucei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
100% aminoacylation of cytosolic tRNA, 4% aminoacylation of mitochondrial tRNA | Trypanosoma brucei |
| additional information | - |
100% aminoacylation of cytosolic tRNA, 90% aminoacylation of mitochondrial tRNA | Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate + tRNAAsp | - |
Trypanosoma brucei | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aspartyl-tRNA synthetase | - |
Trypanosoma brucei |
| AspRS | - |
Trypanosoma brucei |
| Tb-AspRS1 | - |
Trypanosoma brucei |
| Tb-AspRS2 | - |
Trypanosoma brucei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Trypanosoma brucei | |
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Release 2023.1 (January 2023)
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