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Literature summary for 6.1.1.12 extracted from
- Systematic analysis of fusion and affinity tags using human aspartyl-tRNA synthetase expressed in E. coli (2007), Protein Expr. Purif., 54, 166-175.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His6-tagged or GST-tagged enzyme in Escherichia coli strain BL21(DE3), and expression of biotin- or His6-tagged DRS-SUMO and DRS-ubiquitin fusion proteins in Escherichia coli strain BL21(DE3), since the free DRS is expressed as an inactive and insoluble protein in Escherichia coli, SUMO is a small ubiquitin-like modifier molecule | Homo sapiens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate + tRNAAsp | Homo sapiens | - |
AMP + diphosphate + aspartyl-tRNAAsp | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His6-tagged DRS-SUMO and DRS-ubiquitin fusion proteins by nickel affinity chromatography from Escherichia coli strain BL21(DE3), recombinant biotin-tagged DRS-ubiquitin fusion protein by avidin affinity chromatography from Escherichia coli strain Bl21(DE3) | Homo sapiens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.7 | - |
purified recombinant His6-tagged DRS enzyme | Homo sapiens |
| 10.9 | - |
purified recombinant His6-tagged DRS-ubiquitin fusion enzyme | Homo sapiens |
| 11.5 | - |
purified recombinant biotin-tagged DRS-ubiquitin fusion enzyme | Homo sapiens |
| 13.5 | - |
purified recombinant His6-tagged DRS-small ubiquitin-like modifier fusion enzyme | Homo sapiens |
| 17 | - |
purified recombinant GST-tagged DRS enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate + tRNAAsp | - |
Homo sapiens | AMP + diphosphate + aspartyl-tRNAAsp | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aspartyl-tRNA synthetase | - |
Homo sapiens |
| DRS | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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