Any feedback?
Literature summary for 6.1.1.12 extracted from
- Aspartyl-tRNA synthetase is the target of peptide nucleotide antibiotic Microcin C (2006), J. Biol. Chem., 281, 18033-18042.
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | the enzyme is the target of peptide nucleotide antibiotic Microcin C | Francisella tularensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene aspS, expression of His-tagged enzyme in Escherichia coli strain Bl21(DE3) | Francisella tularensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Microcin C | a ribosome-synthesized heptapeptide that contains a modified adenosine monophosphate covalently attached to the C-terminal aspartate, a potent inhibitor of bacterial growth, that targets the translation process via its degradation product, a modified aspartyl-adenylate containing an N-acylphosphoramidate linkage, overview, Microcystin is recombinantly expressed in Escherichia coli strain TG1 | Francisella tularensis |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Francisella tularensis | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate + tRNAAsp | Francisella tularensis | - |
AMP + diphosphate + aspartyl-tRNAAsp | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Francisella tularensis | - |
gene aspS | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Francisella tularensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-aspartate + tRNAAsp | - |
Francisella tularensis | AMP + diphosphate + aspartyl-tRNAAsp | - |
? | |
| additional information | in vitro transcription-translation activity and inhibition by Microcystin C, overview | Francisella tularensis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Aspartyl-tRNA synthetase | - |
Francisella tularensis |
| AspRS | - |
Francisella tularensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Francisella tularensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Francisella tularensis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Francisella tularensis | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Microcystin C in vivo inhibition kinetics | Francisella tularensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
