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Literature summary for 6.1.1.12 extracted from

  • Feng, L.; Tumbula-Hansen, D.; Toogood, H.; Soll, D.
    Expanding tRNA recognition of a tRNA synthetase by a single amino acid change (2003), Proc. Natl. Acad. Sci. USA, 100, 5676-5681.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.12

Protein Variants

Protein Variants Comment Organism
K85P discriminating AspRS gains the ability to form Asp-tRNAAsn. Mutation impairs the ability to synthesize Asp-trNAASp in vitr, 8fold increase in KM-value for tRNAAsp Thermococcus kodakarensis
K85P the wild-type enzyme shows no activity with tRNAAsn, the mutant is active with tRNAAsn Thermococcus kodakarensis
W26H discriminating AspRS gains the ability to form Asp-tRNAAsn. Mutation causes a 1.5fold decrease in overall catalytic efficiency for Asp-tRNAASp synthesis Thermococcus kodakarensis
W26H the wild-type enzyme shows no activity with tRNAAsn, the mutant is active with tRNAAsn Thermococcus kodakarensis
W26H/K85P discriminating AspRS gains the ability to form Asp-tRNAAsn Thermococcus kodakarensis
W26H/K85P the wild-type enzyme shows no activity with tRNAAsn, the mutant is active with tRNAAsn Thermococcus kodakarensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 tRNAAsp pH 6.0, 60°C, wild-type enzyme Thermococcus kodakarensis
0.0012
-
 tRNAAsp pH 6.0, 60°C, mutant enzyme W26HK85P Thermococcus kodakarensis
0.0014
-
 tRNAAsp pH 6.0, 60°C, wild-type enzyme Thermococcus kodakarensis
0.0031
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme K85P Thermococcus kodakarensis
0.0031
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme W26H/K85P Thermococcus kodakarensis
0.0045
-
 tRNAAsp pH 6.0, 60°C, mutant enzyme W26H Thermococcus kodakarensis
0.0062
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme W26H/K85P Thermococcus kodakarensis
0.0062
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme W26HK85P Thermococcus kodakarensis
0.0091
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme K85P Thermococcus kodakarensis
0.0091
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme W26H Thermococcus kodakarensis
0.011
-
 tRNAAsp pH 6.0, 60°C, mutant enzyme K85P Thermococcus kodakarensis

Organism

Organism UniProt Comment Textmining
Thermococcus kodakarensis
-
-
-
Thermococcus kodakarensis Q52428
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Asp + tRNAAsn discriminating AspRS gains the ability to form Asp-tRNAAsn in vitro when the W26H or K85P changes are introduced independently or in combination Thermococcus kodakarensis AMP + diphosphate + aspartyl-tRNAAsn
-
 ?
ATP + Asp + tRNAAsp
-
 Thermococcus kodakarensis AMP + diphosphate + aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsn wild-type enzyme shows no activity with tRNAAsn. Mutant enzymes W26H, K85P and W26H/K85P are active with tRNAAsn Thermococcus kodakarensis AMP + diphosphate + L-aspartyl-tRNAAsn
-
 ?
ATP + L-aspartate + tRNAAsp wild-type enzyme shows no activity with tRNAAsn Thermococcus kodakarensis AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?

Synonyms

Synonyms Comment Organism
AspRS
-
 Thermococcus kodakarensis
D-AspRS
-
 Thermococcus kodakarensis
discriminating aspartyl-tRNA synthetase
-
 Thermococcus kodakarensis
discriminating AspRS
-
 Thermococcus kodakarensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermococcus kodakarensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.009
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme K85P Thermococcus kodakarensis
0.009
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme W26H/K85P Thermococcus kodakarensis
0.026
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme K85P Thermococcus kodakarensis
0.026
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme W26H Thermococcus kodakarensis
0.034
-
 tRNAAsp pH 6.0, 60°C, mutant enzyme W26H/K85P Thermococcus kodakarensis
0.034
-
 tRNAAsp pH 6.0, 60°C, mutant enzyme W26HK85P Thermococcus kodakarensis
0.037
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme W26H/K85P Thermococcus kodakarensis
0.037
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme W26HK85P Thermococcus kodakarensis
0.061
-
 tRNAAsp pH 6.0, 60°C, mutant enzyme K85P Thermococcus kodakarensis
0.061
-
 tRNAAsp pH 6.0, 60°C, wild-type enzyme Thermococcus kodakarensis
0.116
-
 tRNAAsp pH 6.0, 60°C, mutant enzyme W26H Thermococcus kodakarensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Thermococcus kodakarensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.9
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme K85P Thermococcus kodakarensis
2.9
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme W26H Thermococcus kodakarensis
5.8
-
 tRNAAsp pH 6.0, 60°C, mutant enzyme K85P Thermococcus kodakarensis
6
-
 tRNAAsn pH 6.0, 60°C, mutant enzyme W26HK85P Thermococcus kodakarensis
26
-
 tRNAAsp pH 6.0, 60°C, mutant enzyme W26H Thermococcus kodakarensis
28
-
 tRNAAsp pH 6.0, 60°C, mutant enzyme W26HK85P Thermococcus kodakarensis
45
-
 tRNAAsp pH 6.0, 60°C, wild-type enzyme Thermococcus kodakarensis