Protein Variants | Comment | Organism |
---|---|---|
K85P | discriminating AspRS gains the ability to form Asp-tRNAAsn. Mutation impairs the ability to synthesize Asp-trNAASp in vitr, 8fold increase in KM-value for tRNAAsp | Thermococcus kodakarensis |
K85P | the wild-type enzyme shows no activity with tRNAAsn, the mutant is active with tRNAAsn | Thermococcus kodakarensis |
W26H | discriminating AspRS gains the ability to form Asp-tRNAAsn. Mutation causes a 1.5fold decrease in overall catalytic efficiency for Asp-tRNAASp synthesis | Thermococcus kodakarensis |
W26H | the wild-type enzyme shows no activity with tRNAAsn, the mutant is active with tRNAAsn | Thermococcus kodakarensis |
W26H/K85P | discriminating AspRS gains the ability to form Asp-tRNAAsn | Thermococcus kodakarensis |
W26H/K85P | the wild-type enzyme shows no activity with tRNAAsn, the mutant is active with tRNAAsn | Thermococcus kodakarensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
tRNAAsp | pH 6.0, 60°C, wild-type enzyme | Thermococcus kodakarensis | |
0.0012 | - |
tRNAAsp | pH 6.0, 60°C, mutant enzyme W26HK85P | Thermococcus kodakarensis | |
0.0014 | - |
tRNAAsp | pH 6.0, 60°C, wild-type enzyme | Thermococcus kodakarensis | |
0.0031 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme K85P | Thermococcus kodakarensis | |
0.0031 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme W26H/K85P | Thermococcus kodakarensis | |
0.0045 | - |
tRNAAsp | pH 6.0, 60°C, mutant enzyme W26H | Thermococcus kodakarensis | |
0.0062 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme W26H/K85P | Thermococcus kodakarensis | |
0.0062 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme W26HK85P | Thermococcus kodakarensis | |
0.0091 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme K85P | Thermococcus kodakarensis | |
0.0091 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme W26H | Thermococcus kodakarensis | |
0.011 | - |
tRNAAsp | pH 6.0, 60°C, mutant enzyme K85P | Thermococcus kodakarensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus kodakarensis | - |
- |
- |
Thermococcus kodakarensis | Q52428 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Asp + tRNAAsn | discriminating AspRS gains the ability to form Asp-tRNAAsn in vitro when the W26H or K85P changes are introduced independently or in combination | Thermococcus kodakarensis | AMP + diphosphate + aspartyl-tRNAAsn | - |
? | |
ATP + Asp + tRNAAsp | - |
Thermococcus kodakarensis | AMP + diphosphate + aspartyl-tRNAAsp | - |
? | |
ATP + L-aspartate + tRNAAsn | wild-type enzyme shows no activity with tRNAAsn. Mutant enzymes W26H, K85P and W26H/K85P are active with tRNAAsn | Thermococcus kodakarensis | AMP + diphosphate + L-aspartyl-tRNAAsn | - |
? | |
ATP + L-aspartate + tRNAAsp | wild-type enzyme shows no activity with tRNAAsn | Thermococcus kodakarensis | AMP + diphosphate + L-aspartyl-tRNAAsp | - |
? |
Synonyms | Comment | Organism |
---|---|---|
AspRS | - |
Thermococcus kodakarensis |
D-AspRS | - |
Thermococcus kodakarensis |
discriminating aspartyl-tRNA synthetase | - |
Thermococcus kodakarensis |
discriminating AspRS | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Thermococcus kodakarensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme K85P | Thermococcus kodakarensis | |
0.009 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme W26H/K85P | Thermococcus kodakarensis | |
0.026 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme K85P | Thermococcus kodakarensis | |
0.026 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme W26H | Thermococcus kodakarensis | |
0.034 | - |
tRNAAsp | pH 6.0, 60°C, mutant enzyme W26H/K85P | Thermococcus kodakarensis | |
0.034 | - |
tRNAAsp | pH 6.0, 60°C, mutant enzyme W26HK85P | Thermococcus kodakarensis | |
0.037 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme W26H/K85P | Thermococcus kodakarensis | |
0.037 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme W26HK85P | Thermococcus kodakarensis | |
0.061 | - |
tRNAAsp | pH 6.0, 60°C, mutant enzyme K85P | Thermococcus kodakarensis | |
0.061 | - |
tRNAAsp | pH 6.0, 60°C, wild-type enzyme | Thermococcus kodakarensis | |
0.116 | - |
tRNAAsp | pH 6.0, 60°C, mutant enzyme W26H | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Thermococcus kodakarensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme K85P | Thermococcus kodakarensis | |
2.9 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme W26H | Thermococcus kodakarensis | |
5.8 | - |
tRNAAsp | pH 6.0, 60°C, mutant enzyme K85P | Thermococcus kodakarensis | |
6 | - |
tRNAAsn | pH 6.0, 60°C, mutant enzyme W26HK85P | Thermococcus kodakarensis | |
26 | - |
tRNAAsp | pH 6.0, 60°C, mutant enzyme W26H | Thermococcus kodakarensis | |
28 | - |
tRNAAsp | pH 6.0, 60°C, mutant enzyme W26HK85P | Thermococcus kodakarensis | |
45 | - |
tRNAAsp | pH 6.0, 60°C, wild-type enzyme | Thermococcus kodakarensis |