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Literature summary for 6.1.1.12 extracted from

  • Becker, H.D.; Reinbolt, J.; Kreutzer, R.; Giege, R.; Kern, D.
    Existence of two distinct aspartyl-tRNA synthetases in Thermus thermophilus. Structural and biochemical properties of the two enzymes (1997), Biochemistry, 36, 8785-8797.
    View publication on PubMed
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km value of pure tRNA, transcript tRNA and unfractionated tRNAs of various origins with enzyme form AspRS1 and enzyme form AspRS2 Thermus thermophilus
0.003
-
 Asp 37°C and 70°C, tRNA aminoacylation, enzyme form AspRS2 Thermus thermophilus
0.009
-
 Asp 37°C, tRNA aminoacylation, enzyme form AspRS1 Thermus thermophilus
0.03
-
 Asp 37°C, ATP-diphosphate exchange, and 70°C, tRNAaminoacylation, enzyme form AspRS1 Thermus thermophilus
0.062
-
 Asp 37°C, ATP-diphosphate exchange, enzyme form AspRS2 Thermus thermophilus
0.1
-
 Asp 70°C, ATP-diphosphate exchange by enzyme form AspRS1, and tRNA aminoacylation by enzyme form AspRS2 Thermus thermophilus
0.12
-
 ATP 37°C, tRNA aminoacylation, enzyme form AspRS1 Thermus thermophilus
0.25
-
 ATP 70°C, ATP-diphosphate exchange, enzyme form AspRS1 Thermus thermophilus
0.28
-
 ATP 70°C, tRNA aminoacylation, enzyme form AspRS1 Thermus thermophilus
0.37
-
 ATP 37°C, ATP-diphosphate exchange, enzyme form AspRS1 Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 distinct enzyme forms: AspRS1 and AspRS2 Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0405
-
 enzyme form AspRS2 Thermus thermophilus
0.0452
-
 enzyme form AspRS1 Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-aspartate + tRNAAsp
-
 Thermus thermophilus AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
ATP + L-aspartate + tRNAAsp
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-aspartyl-tRNAAsp
-
 ?
additional information aspartate-dependent ATP-diphosphate exchange Thermus thermophilus ?
-
 ?
additional information aspartate-dependent ATP-diphosphate exchange Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?