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Literature summary for 6.1.1.11 extracted from

  • Weygand-Durasevic, I.; Lenard, B.; Filipic, S.; Söll, D.
    The C-terminal extension of yeast seryl-tRNA synthetase affects stability of the enzyme and its substrate affinity (1996), J. Biol. Chem., 271, 2455-2461.
    View publication on PubMed
Search for more literature for 6.1.1.11

Protein Variants

Protein Variants Comment Organism
additional information overexpressing, mutant lacking those 60 base pairs that encode the C-terminal peptide. The truncation mutant is less stable to heat inactivation at 42°C and binds tRNA with 3.6fold higher affinity, while the Km for Ser is 4fold increased relatively to the wild-type Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00005
-
 tRNASer aminoacylation, truncated enzyme Saccharomyces cerevisiae
0.00019
-
 tRNASer aminoacylation, wild-type enzyme Saccharomyces cerevisiae
0.0143
-
 ATP aminoacylation, truncated enzyme Saccharomyces cerevisiae
0.021
-
 ATP aminoacylation, wild-type enzyme Saccharomyces cerevisiae
0.0625
-
 Ser aminoacylation, wild-type enzyme Saccharomyces cerevisiae
0.252
-
 Ser aminoacylation, truncated enzyme Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 overexpression, wild-type and mutant lacking the 60 base pairs encoding the C-terminal peptide
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
 Saccharomyces cerevisiae AMP + diphosphate + L-seryl-tRNASer
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
42
-
 30 min, about 80% loss of activity of full-length enzyme, about 5% loss of activity of truncated enzyme Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.41
-
 tRNASer Ser, , aminoacylation, truncated enzyme Saccharomyces cerevisiae
0.5
-
 Ser ATP, , aminoacylation, wild-type enzyme Saccharomyces cerevisiae
0.52
-
 ATP aminoacylation, truncated enzyme Saccharomyces cerevisiae
0.55
-
 tRNASer aminoacylation, wild-type enzyme Saccharomyces cerevisiae