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Literature summary for 6.1.1.11 extracted from
- Exclusive cytosolic localization and broad tRNASer specificity of Arabidopsis thaliana seryl-tRNA synthetase (2016), J. Plant Biol., 59, 44-54 .
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Arabidopsis thaliana |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000759 | - |
tRNASer (Escherichia coli) | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.000937 | - |
tRNASer (Saccharomyces cerevisiae) | at pH 7.5 and 30°C | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Arabidopsis thaliana | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | Arabidopsis thaliana | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
ecotype Columbia | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA agarose column chromatography, MonoQ colun chromatography, and Superdex 200 gel filtration | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Arabidopsis thaliana | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | - |
Arabidopsis thaliana | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
| ATP + L-serine + tRNASer (Escherichia coli) | - |
Arabidopsis thaliana | AMP + diphosphate + L-seryl-tRNASer (Escherichia coli) | - |
? | |
| ATP + L-serine + tRNASer (Saccharomyces cerevisiae) | - |
Arabidopsis thaliana | AMP + diphosphate + L-seryl-tRNASer (Saccharomyces cerevisiae) | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, green fluorescent protein-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SerRS | - |
Arabidopsis thaliana |
| Seryl-tRNA synthetase | - |
Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
tRNASer (Saccharomyces cerevisiae) | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 0.14 | - |
tRNASer (Escherichia coli) | at pH 7.5 and 30°C | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Arabidopsis thaliana | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 140 | - |
tRNASer (Saccharomyces cerevisiae) | at pH 7.5 and 30°C | Arabidopsis thaliana | |
| 180 | - |
tRNASer (Escherichia coli) | at pH 7.5 and 30°C | Arabidopsis thaliana |
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Release 2023.1 (January 2023)
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