Protein Variants | Comment | Organism |
---|---|---|
F397P/A399G | the mutant shows deleterious effects in both kcat and KM values compared to the wild type enzyme | Methanosarcina barkeri |
G402A/G405A | the double substitution displays an order of magnitude higher KM but only 2.5fold lower kcat | Methanosarcina barkeri |
H250A | the mutant is highly efficient in serine activation: kcat is reduced by only 3fold, while KM is reduced relative to the wild type parameters | Methanosarcina barkeri |
H250A/Q400A | the mutant is highly efficient in serine activation: kcat is reduced by only 3fold, while KM is reduced relative to the wild type parameters | Methanosarcina barkeri |
P395A | the mutant shows 6.5fold reduced enzyme efficiency compared to the wild type | Methanosarcina barkeri |
Q400A | the mutation produces only small kinetic effects: KM is elevated 2fold while kcat is reduced 2fold | Methanosarcina barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
L-serine | wild type enzyme, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.0257 | - |
L-serine | mutant enzyme P395A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.049 | - |
L-serine | mutant enzyme Q400A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.0731 | - |
L-serine | mutant enzyme H250A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.2491 | - |
L-serine | mutant enzyme G402A/G405A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.9 | - |
L-serine | mutant enzyme F397P/A399G, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | Methanosarcina barkeri | - |
AMP + diphosphate + L-seryl-tRNASer | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Methanosarcina barkeri | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SerRS | - |
Methanosarcina barkeri |
Seryl-tRNA synthetase | - |
Methanosarcina barkeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0014 | - |
L-serine | mutant enzyme H250A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.016 | - |
L-serine | mutant enzyme F397P/A399G, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.15 | - |
L-serine | mutant enzyme P395A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.38 | - |
L-serine | mutant enzyme Q400A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.41 | - |
L-serine | mutant enzyme G402A/G405A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
1.06 | - |
L-serine | wild type enzyme, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methanosarcina barkeri |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
L-serine | wild type enzyme, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.0257 | - |
L-serine | mutant enzyme P395A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.049 | - |
L-serine | mutant enzyme Q400A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.0731 | - |
L-serine | mutant enzyme H250A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.2491 | - |
L-serine | mutant enzyme G402A/G405A, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri | |
0.9 | - |
L-serine | mutant enzyme F397P/A399G, in 50 mM HEPES pH 7.0, 15 mM MgCl2, 40 mM KCl, at 37°C | Methanosarcina barkeri |