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Literature summary for 6.1.1.11 extracted from

  • Rokov-Plavec, J.; Lesjak, S.; Gruic-Sovulj, I.; Mocibob, M.; Dulic, M.; Weygand-Durasevic, I.
    Substrate recognition and fidelity of maize seryl-tRNA synthetases (2013), Arch. Biochem. Biophys., 529, 122-130.
    View publication on PubMed
Search for more literature for 6.1.1.11

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli strain KL229 Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008628
-
 tRNASer in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
0.18
-
 L-serine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
2 3.4 L-threonine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
2.35
-
 DL-Serine hydroxamate in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
29
-
 L-cysteine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol the enzyme is localized exclusively in the cytosol Zea mays 5829
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Zea mays
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?
additional information Zea mays the enzyme shows pre-transfer editing activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + DL-serine hydroxamate + tRNASer very weak activity Zea mays ?
-
 ?
ATP + L-cysteine + tRNASer very weak activity Zea mays AMP + diphosphate + L-cysteinyl-tRNASer
-
 ?
ATP + L-serine + tRNASer
-
 Zea mays AMP + diphosphate + L-seryl-tRNASer
-
 ?
ATP + L-serine + tRNASer tRNASer from Escherichia coli Zea mays AMP + diphosphate + L-seryl-tRNASer
-
 ?
ATP + L-threonine + tRNASer very weak activity Zea mays AMP + diphosphate + L-threonyl-tRNASer
-
 ?
additional information the enzyme shows pre-transfer editing activity Zea mays ?
-
 ?
additional information Gly73 is a major identity element recognized by the enzyme Zea mays ?
-
 ?

Synonyms

Synonyms Comment Organism
SerRS
-
 Zea mays
Seryl-tRNA synthetase
-
 Zea mays

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.078
-
 L-cysteine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
0.14
-
 L-threonine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
1.1
-
 tRNASer in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
2.433
-
 DL-Serine hydroxamate in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
2.5
-
 L-serine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Zea mays

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0027
-
 L-cysteine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
0.006
-
 L-threonine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
1.04
-
 DL-Serine hydroxamate in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
13.9
-
 L-serine in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays
1275
-
 tRNASer in 50 mM HEPES pH 7.0, 25 mM KCl, 20 mM MgCl2, temperature not specified in the publication Zea mays