Literature summary for 6.1.1.11 extracted from

Rocha, R.; Barbosa Pereira, P.J.; Santos, M.A.; Macedo-Ribeiro, S.
Purification, crystallization and preliminary X-ray diffraction analysis of the seryl-tRNA synthetase from Candida albicans (2011), Acta Crystallogr. Sect. F, 67, 153-156.

Cloned(Commentary)

Cloned (Comment)	Organism
large-scale expression of His-tagged isozymes in Escherichia coli strain BL21 (DE3)	Candida albicans

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified isozymes, sitting-drop vapour-diffusion method, 8-14 mg/ml protein in 50 mM Na HEPES, pH 7.6, 150 mM KCl, 10 mM MgCl2, and 8% v/v glycerol, is mixed with reservoir solution containing 100 mM Na MES pH 5.6-5.8, 3.2-3.4 M ammonium sulfate and 0-2% v/v glycerol, equilibration against 0.5 ml of reservoir solution. Binary complexes SerRS-SerSA and SerRS-ATP, SerRS_Ser197 with 15 mM 5'-O-[N-(l-seryl)sulfamoyl]adenosine or 10 mM ATP, are mixed with 100 mM Na MES pH 5.8-6.2, 3.3-3.4 M ammonium sulfate and 0-5% v/v glycerol as precipitant solution, 2-3 days, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement for analysis of crystal structures of unliganded SerRS and of its complexes with ATP and with a seryl-adenylate analogue	Candida albicans

Crystallization (Comment)

Organism

purified isozymes, sitting-drop vapour-diffusion method, 8-14 mg/ml protein in 50 mM Na HEPES, pH 7.6, 150 mM KCl, 10 mM MgCl2, and 8% v/v glycerol, is mixed with reservoir solution containing 100 mM Na MES pH 5.6-5.8, 3.2-3.4 M ammonium sulfate and 0-2% v/v glycerol, equilibration against 0.5 ml of reservoir solution. Binary complexes SerRS-SerSA and SerRS-ATP, SerRS_Ser197 with 15 mM 5'-O-[N-(l-seryl)sulfamoyl]adenosine or 10 mM ATP, are mixed with 100 mM Na MES pH 5.8-6.2, 3.3-3.4 M ammonium sulfate and 0-5% v/v glycerol as precipitant solution, 2-3 days, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement for analysis of crystal structures of unliganded SerRS and of its complexes with ATP and with a seryl-adenylate analogue

Candida albicans

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytoplasm	-	Candida albicans	5737	-

Organism

Organism	UniProt	Comment	Textmining
Candida albicans	Q9HGT6	two isozymes through poylmorphism at position 197, L197S	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged isozymes from Escherichia coli strain BL21 (DE3) by nickel affinity and anion exchange chromatography, followed by ultrafiltration and gel filtration	Candida albicans

Subunits

Subunits	Comment	Organism
dimer	three-dimensional structure analysis, overview	Candida albicans

Synonyms

Synonyms	Comment	Organism
SerRS	-	Candida albicans
Seryl-tRNA synthetase	-	Candida albicans