Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 6.1.1.11 extracted from

  • Bilokapic, S.; Ivic, N.; Godinic-Mikulcic, V.; Piantanida, I.; Ban, N.; Weygand-Durasevic, I.
    Idiosyncratic helix-turn-helix motif in Methanosarcina barkeri seryl-tRNA synthetase has a critical architectural role (2009), J. Biol. Chem., 284, 10706-10713.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.11

Cloned(Commentary)

Cloned (Comment) Organism
the vectors pET15b, pET20b, and pET28b are used for expression of the proteins in Escherichia coli BL21 cells, the vectors pACT2 and pAB151 are used in yeast two-hybrid analysis Methanosarcina barkeri

Protein Variants

Protein Variants Comment Organism
E273A E273A replacement in helix 9 located in the C-terminal domain Methanosarcina barkeri
E273A/D277A/K280A triple replacement in helix 9 Methanosarcina barkeri
K164A K164A replacement in the linker peptide Methanosarcina barkeri
mMbSerRS-CTD START codon introduced at position 167, which generates the C-terminal domain Methanosarcina barkeri
mMbSerRS-CTDDELTAHTH START codon introduced at position 167 of mMbSerRS-DELTAHTH, generating the C-terminal domain without the helix-turn-helix motif Methanosarcina barkeri
mMbSerRS-DELTAHTH deleted helix-turn-helix motif Methanosarcina barkeri
mMbSerRS-NTD STOP codon introduced at position 164, which generates the N-terminal domain Methanosarcina barkeri
R147A R147A replacement in helix 4 located in the N-terminal domain Methanosarcina barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0024
-
 tRNASer mutant K164A Methanosarcina barkeri
0.0026
-
 tRNASer wild-type Methanosarcina barkeri
0.0042
-
 tRNASer mutant E273A Methanosarcina barkeri
0.0047
-
 tRNASer mutant E273A/D277A/K280A Methanosarcina barkeri
0.0146
-
 tRNASer mutant R147A Methanosarcina barkeri

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+
-
 Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-serine + tRNASer Methanosarcina barkeri
-
 AMP + diphosphate + L-seryl-tRNASer
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
 Methanosarcina barkeri AMP + diphosphate + L-seryl-tRNASer
-
 ?

Subunits

Subunits Comment Organism
homodimer
-
 Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
methanogenic-type SerRS
-
 Methanosarcina barkeri
mMbSerRS
-
 Methanosarcina barkeri
SerRS
-
 Methanosarcina barkeri
Seryl-tRNA synthetase
-
 Methanosarcina barkeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0087
-
 tRNASer mutant K164A Methanosarcina barkeri
0.15
-
 tRNASer mutant R147A Methanosarcina barkeri
0.45
-
 tRNASer wild-type Methanosarcina barkeri
0.55
-
 tRNASer mutant E273A Methanosarcina barkeri
1.4
-
 tRNASer mutant E273A/D277A/K280A Methanosarcina barkeri

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Methanosarcina barkeri