Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Methanosarcina barkeri |
Protein Variants | Comment | Organism |
---|---|---|
E338A | Km (mM)(tRNASer): 0.0021, kcat (1/sec) (tRNASer): 0.059 | Methanosarcina barkeri |
G340V/G341A | Km (mM)(tRNASer): 0.0067, kcat (1/sec) (tRNASer): 0.000000011 | Methanosarcina barkeri |
N435A | Km (mM) (L-Ser): 0.07028, kcat (1/sec) (L-Ser): 0.172, Km (mM)(tRNASer): 0.283, kcat (1/sec) (tRNASer): 0.435 | Methanosarcina barkeri |
N435A/S437A | Km (mM) (L-Ser): 0.689, kcat (1/sec) (L-Ser): 0.576, Km (mM)(tRNASer): 0.509, kcat (1/sec) (tRNASer): 0.361 | Methanosarcina barkeri |
N435A/S437A/W396A | Km (mM) (L-Ser): 0.2879, kcat (1/sec) (L-Ser): 1.32, Km (mM)(tRNASer): 1.191, kcat (1/sec) (tRNASer): 0.175 | Methanosarcina barkeri |
R267A | Km (mM)(tRNASer): 0.0034, kcat (1/sec) (tRNASer): 0.032 | Methanosarcina barkeri |
R347A | Km (mM)(tRNASer): 0.0063, kcat (1/sec) (tRNASer): 0.0026 | Methanosarcina barkeri |
S437A | Km (mM) (L-Ser): 0.573, kcat (1/sec) (L-Ser): 0.729, Km (mM)(tRNASer): 0.412, kcat (1/sec) (tRNASer): 0.396 | Methanosarcina barkeri |
W396A | Km (mM)(L-Ser): 0.2645, kcat (1/sec) (L-Ser): 0.287, Km (mM)(tRNASer): 0.760, kcat (1/sec) (tRNASer): 0.239 | Methanosarcina barkeri |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alanine | - |
Methanosarcina barkeri | |
cysteine | - |
Methanosarcina barkeri | |
glycine | - |
Methanosarcina barkeri | |
threonine | - |
Methanosarcina barkeri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0021 | - |
tRNASer | mutant E338A | Methanosarcina barkeri | |
0.0029 | - |
tRNASer | wild-type | Methanosarcina barkeri | |
0.0034 | - |
tRNASer | mutant R267A | Methanosarcina barkeri | |
0.0061 | - |
L-Ser | wild-type | Methanosarcina barkeri | |
0.0063 | - |
tRNASer | mutant R347A | Methanosarcina barkeri | |
0.0067 | - |
tRNASer | mutant G340V/G341A | Methanosarcina barkeri | |
0.01113 | - |
tRNASer | wild-type | Methanosarcina barkeri | |
0.07028 | - |
L-Ser | mutant N435A | Methanosarcina barkeri | |
0.264 | - |
L-Ser | mutant W396A | Methanosarcina barkeri | |
0.283 | - |
tRNASer | mutant N435A | Methanosarcina barkeri | |
0.412 | - |
tRNASer | mutant S437A | Methanosarcina barkeri | |
0.509 | - |
tRNASer | mutant N435A/S437A | Methanosarcina barkeri | |
0.573 | - |
L-Ser | mutant S437A | Methanosarcina barkeri | |
0.689 | - |
L-Ser | mutant N435A/S437A | Methanosarcina barkeri | |
0.76 | - |
tRNASer | mutant W396A | Methanosarcina barkeri | |
1.191 | - |
tRNASer | mutant N435A/S437A/W396A | Methanosarcina barkeri | |
2.879 | - |
L-Ser | mutant N435A/S437A/W396A | Methanosarcina barkeri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina barkeri | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-serine + tRNASer | - |
Methanosarcina barkeri | AMP + diphosphate + L-seryl-tRNASer | - |
? |
Synonyms | Comment | Organism |
---|---|---|
methanogenic-type SerRSs | - |
Methanosarcina barkeri |
mMbSerRS | - |
Methanosarcina barkeri |
Seryl-tRNA synthetase | - |
Methanosarcina barkeri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Methanosarcina barkeri |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000000011 | - |
tRNASer | mutant G340V/G341A | Methanosarcina barkeri | |
0.00026 | - |
tRNASer | mutant R347A | Methanosarcina barkeri | |
0.032 | - |
tRNASer | mutant R267A | Methanosarcina barkeri | |
0.059 | - |
tRNASer | mutant E338A | Methanosarcina barkeri | |
0.172 | - |
L-Ser | mutant N435A | Methanosarcina barkeri | |
0.175 | - |
tRNASer | mutant N435A/S437A/W396A | Methanosarcina barkeri | |
0.239 | - |
tRNASer | mutant W396A | Methanosarcina barkeri | |
0.287 | - |
L-Ser | mutant W396A | Methanosarcina barkeri | |
0.361 | - |
tRNASer | mutant N435A/S437A | Methanosarcina barkeri | |
0.396 | - |
tRNASer | mutant S437A | Methanosarcina barkeri | |
0.435 | - |
tRNASer | mutant N435A | Methanosarcina barkeri | |
0.454 | - |
tRNASer | wild-type | Methanosarcina barkeri | |
0.473 | - |
tRNASer | wild-type | Methanosarcina barkeri | |
0.576 | - |
L-Ser | mutant N435A/S437A | Methanosarcina barkeri | |
0.729 | - |
L-Ser | mutant S437A | Methanosarcina barkeri | |
1.32 | - |
L-Ser | mutant N435A/S437A/W396A | Methanosarcina barkeri | |
2.06 | - |
L-Ser | wild-type | Methanosarcina barkeri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Methanosarcina barkeri |