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Literature summary for 6.1.1.11 extracted from

  • Bilokapic, S.; Rokov Plavec, J.; Ban, N.; Weygand-Durasevic, I.
    Structural flexibility of the methanogenic-type seryl-tRNA synthetase active site and its implication for specific substrate recognition (2008), FEBS J., 275, 2831-2844.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Methanosarcina barkeri

Protein Variants

Protein Variants Comment Organism
E338A Km (mM)(tRNASer): 0.0021, kcat (1/sec) (tRNASer): 0.059 Methanosarcina barkeri
G340V/G341A Km (mM)(tRNASer): 0.0067, kcat (1/sec) (tRNASer): 0.000000011 Methanosarcina barkeri
N435A Km (mM) (L-Ser): 0.07028, kcat (1/sec) (L-Ser): 0.172, Km (mM)(tRNASer): 0.283, kcat (1/sec) (tRNASer): 0.435 Methanosarcina barkeri
N435A/S437A Km (mM) (L-Ser): 0.689, kcat (1/sec) (L-Ser): 0.576, Km (mM)(tRNASer): 0.509, kcat (1/sec) (tRNASer): 0.361 Methanosarcina barkeri
N435A/S437A/W396A Km (mM) (L-Ser): 0.2879, kcat (1/sec) (L-Ser): 1.32, Km (mM)(tRNASer): 1.191, kcat (1/sec) (tRNASer): 0.175 Methanosarcina barkeri
R267A Km (mM)(tRNASer): 0.0034, kcat (1/sec) (tRNASer): 0.032 Methanosarcina barkeri
R347A Km (mM)(tRNASer): 0.0063, kcat (1/sec) (tRNASer): 0.0026 Methanosarcina barkeri
S437A Km (mM) (L-Ser): 0.573, kcat (1/sec) (L-Ser): 0.729, Km (mM)(tRNASer): 0.412, kcat (1/sec) (tRNASer): 0.396 Methanosarcina barkeri
W396A Km (mM)(L-Ser): 0.2645, kcat (1/sec) (L-Ser): 0.287, Km (mM)(tRNASer): 0.760, kcat (1/sec) (tRNASer): 0.239 Methanosarcina barkeri

Inhibitors

Inhibitors Comment Organism Structure
alanine
-
Methanosarcina barkeri
cysteine
-
Methanosarcina barkeri
glycine
-
Methanosarcina barkeri
threonine
-
Methanosarcina barkeri

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0021
-
tRNASer mutant E338A Methanosarcina barkeri
0.0029
-
tRNASer wild-type Methanosarcina barkeri
0.0034
-
tRNASer mutant R267A Methanosarcina barkeri
0.0061
-
L-Ser wild-type Methanosarcina barkeri
0.0063
-
tRNASer mutant R347A Methanosarcina barkeri
0.0067
-
tRNASer mutant G340V/G341A Methanosarcina barkeri
0.01113
-
tRNASer wild-type Methanosarcina barkeri
0.07028
-
L-Ser mutant N435A Methanosarcina barkeri
0.264
-
L-Ser mutant W396A Methanosarcina barkeri
0.283
-
tRNASer mutant N435A Methanosarcina barkeri
0.412
-
tRNASer mutant S437A Methanosarcina barkeri
0.509
-
tRNASer mutant N435A/S437A Methanosarcina barkeri
0.573
-
L-Ser mutant S437A Methanosarcina barkeri
0.689
-
L-Ser mutant N435A/S437A Methanosarcina barkeri
0.76
-
tRNASer mutant W396A Methanosarcina barkeri
1.191
-
tRNASer mutant N435A/S437A/W396A Methanosarcina barkeri
2.879
-
L-Ser mutant N435A/S437A/W396A Methanosarcina barkeri

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-serine + tRNASer
-
Methanosarcina barkeri AMP + diphosphate + L-seryl-tRNASer
-
?

Synonyms

Synonyms Comment Organism
methanogenic-type SerRSs
-
Methanosarcina barkeri
mMbSerRS
-
Methanosarcina barkeri
Seryl-tRNA synthetase
-
Methanosarcina barkeri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Methanosarcina barkeri

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000000011
-
tRNASer mutant G340V/G341A Methanosarcina barkeri
0.00026
-
tRNASer mutant R347A Methanosarcina barkeri
0.032
-
tRNASer mutant R267A Methanosarcina barkeri
0.059
-
tRNASer mutant E338A Methanosarcina barkeri
0.172
-
L-Ser mutant N435A Methanosarcina barkeri
0.175
-
tRNASer mutant N435A/S437A/W396A Methanosarcina barkeri
0.239
-
tRNASer mutant W396A Methanosarcina barkeri
0.287
-
L-Ser mutant W396A Methanosarcina barkeri
0.361
-
tRNASer mutant N435A/S437A Methanosarcina barkeri
0.396
-
tRNASer mutant S437A Methanosarcina barkeri
0.435
-
tRNASer mutant N435A Methanosarcina barkeri
0.454
-
tRNASer wild-type Methanosarcina barkeri
0.473
-
tRNASer wild-type Methanosarcina barkeri
0.576
-
L-Ser mutant N435A/S437A Methanosarcina barkeri
0.729
-
L-Ser mutant S437A Methanosarcina barkeri
1.32
-
L-Ser mutant N435A/S437A/W396A Methanosarcina barkeri
2.06
-
L-Ser wild-type Methanosarcina barkeri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
assay at Methanosarcina barkeri