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Literature summary for 6.1.1.11 extracted from
- Trypanosoma seryl-tRNA synthetase is a metazoan-like enzyme with high affinity for tRNASec (2006), J. Biol. Chem., 281, 38217-38225.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene encoding SerRS, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis, expression of His-tagged SerRS in Escherichia coli | Trypanosoma cruzi |
| gene SerS, DNA and amino acid sequence determination and analysis, sequence comparison, phylogenetic analysis | Trypanosoma brucei |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | RNAi-mediated ablation of SerRS using stem-loop constructs containing the puromycin resistance gene, overview | Trypanosoma brucei |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | Trypanosoma brucei is highly sensitive to the action of specific selenoprotein inhibitors | Trypanosoma brucei |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Trypanosoma brucei | |
| 0.0004 | - |
tRNASecUCA | pH 7.6, 37°C, recombinant enzyme | Trypanosoma brucei | |
| 0.0032 | - |
tRNASerCGA | pH 7.6, 37°C, recombinant enzyme | Trypanosoma brucei |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Trypanosoma brucei |  |
| Mg2+ | - |
Trypanosoma cruzi | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASec | Trypanosoma brucei | SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins, SerRS is essential for growth Trypanosoma brucei and is responsible for the serylation of both tRNASer and tRNASec | AMP + diphosphate + L-seryl-tRNASec | - |
? | |
| ATP + L-serine + tRNASec | Trypanosoma cruzi | SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins, SerRS is responsible for the serylation of both tRNASer and tRNASec | AMP + diphosphate + L-seryl-tRNASec | - |
? | |
| ATP + L-serine + tRNASer | Trypanosoma cruzi | responsible for the serylation of both tRNASer and tRNASec | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
| ATP + L-serine + tRNASer | Trypanosoma brucei | tRNASec, unlike most trypanosomal tRNAs, is exclusively localized in the cytosol, SerRS is essential for growth Trypanosoma brucei and is responsible for the serylation of both tRNASer and tRNASec | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
gene SerS | - |
| Trypanosoma cruzi | Q4CW46 | fragment; gene SerS | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged SerRS from Escherichia coli by nickel affinity chromatography | Trypanosoma cruzi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASec | SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins, SerRS is essential for growth Trypanosoma brucei and is responsible for the serylation of both tRNASer and tRNASec | Trypanosoma brucei | AMP + diphosphate + L-seryl-tRNASec | - |
? | |
| ATP + L-serine + tRNASec | SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins, SerRS is responsible for the serylation of both tRNASer and tRNASec | Trypanosoma cruzi | AMP + diphosphate + L-seryl-tRNASec | - |
? | |
| ATP + L-serine + tRNASec | SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins | Trypanosoma cruzi | AMP + diphosphate + L-seryl-tRNASec | - |
? | |
| ATP + L-serine + tRNASecUCA | SerRS also aminoacylates tRNASec with serine as the first step for the incorporation of selenocysteine into proteins | Trypanosoma brucei | AMP + diphosphate + L-seryl-tRNASecUCA | - |
? | |
| ATP + L-serine + tRNASer | - |
Trypanosoma cruzi | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
| ATP + L-serine + tRNASer | responsible for the serylation of both tRNASer and tRNASec | Trypanosoma cruzi | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
| ATP + L-serine + tRNASer | tRNASec, unlike most trypanosomal tRNAs, is exclusively localized in the cytosol, SerRS is essential for growth Trypanosoma brucei and is responsible for the serylation of both tRNASer and tRNASec | Trypanosoma brucei | AMP + diphosphate + L-seryl-tRNASer | - |
? | |
| ATP + L-serine + tRNASerCGA | - |
Trypanosoma brucei | AMP + diphosphate + L-seryl-tRNASerCGA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SerRS | - |
Trypanosoma brucei |
| SerRS | - |
Trypanosoma cruzi |
| Seryl-tRNA synthetase | - |
Trypanosoma brucei |
| Seryl-tRNA synthetase | - |
Trypanosoma cruzi |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Trypanosoma brucei |
| 37 | - |
assay at | Trypanosoma cruzi |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Trypanosoma brucei |
| 7.6 | - |
assay at | Trypanosoma cruzi |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Trypanosoma brucei | |
| ATP | - |
Trypanosoma cruzi | |
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Release 2023.1 (January 2023)
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