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Literature summary for 6.1.1.11 extracted from
- Biochemical characterization of VlmL, a Seryl-tRNA synthetase encoded by the valanimycin biosynthetic gene cluster (2006), J. Biol. Chem., 281, 26785-26791.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene serS, phylogenetic analysis | Streptomyces coelicolor |
| gene serS2, phylogenetic analysis | Streptomyces avermitilis |
| genes vlmL and svsR, DNA and amino acid sequence determination and analysis from strain MG456-hF10, phylogenetic analysis, overexpression of soluble VlmL and SvsR in Escherichia coli strain BL21(DE3) | Streptomyces viridifaciens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Streptomyces viridifaciens |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Streptomyces coelicolor |  |
| Mg2+ | - |
Streptomyces avermitilis | |
| Mg2+ | - |
Streptomyces viridifaciens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | Streptomyces coelicolor | - |
AMP + diphosphate + L-seryl-tRNASer | - |
r | |
| ATP + L-serine + tRNASer | Streptomyces avermitilis | - |
AMP + diphosphate + L-seryl-tRNASer | - |
r | |
| ATP + L-serine + tRNASer | Streptomyces viridifaciens | - |
AMP + diphosphate + L-seryl-tRNASer | - |
r | |
| additional information | Streptomyces viridifaciens | SerRS represents the housekeeping seryl-tRNA synthetase in the organism, one role of protein VlmL in valanimycin biosynthesis is to produce seryl-tRNA, which is then utilized for a subsequent step in the biosynthetic pathway, biosynthetic pathway for the antibiotic valanimycin, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces avermitilis | Q82KS8 | gene serS2 | - |
| Streptomyces coelicolor | Q9ZBX1 | gene serS | - |
| Streptomyces viridifaciens | Q84F26 | a valanimycin producing organism, strain MG456-hF10, genes vlmL and svsR, the latter is a housekeeping gene, gene SerRS | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-serine + tRNASer | - |
Streptomyces coelicolor | AMP + diphosphate + L-seryl-tRNASer | - |
r | |
| ATP + L-serine + tRNASer | - |
Streptomyces avermitilis | AMP + diphosphate + L-seryl-tRNASer | - |
r | |
| ATP + L-serine + tRNASer | - |
Streptomyces viridifaciens | AMP + diphosphate + L-seryl-tRNASer | - |
r | |
| additional information | SerRS represents the housekeeping seryl-tRNA synthetase in the organism, one role of protein VlmL in valanimycin biosynthesis is to produce seryl-tRNA, which is then utilized for a subsequent step in the biosynthetic pathway, biosynthetic pathway for the antibiotic valanimycin, overview | Streptomyces viridifaciens | ? | - |
? | |
| additional information | proteins VlmL and SvsR, both involved in valanimycin biosynthesis, are able to catalyze a serine-dependent exchange of diphosphate into ATP and to aminoacylate total Escherichia coli tRNA with L-serine, SvsR is catalytically more efficient than VlmL, overview | Streptomyces viridifaciens | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SerRS | - |
Streptomyces coelicolor |
| SerRS | - |
Streptomyces viridifaciens |
| SerRS2 | - |
Streptomyces avermitilis |
| Seryl-tRNA synthetase | - |
Streptomyces coelicolor |
| Seryl-tRNA synthetase | - |
Streptomyces viridifaciens |
| SvsR | - |
Streptomyces viridifaciens |
| VlmL | - |
Streptomyces viridifaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
ATP-diphosphate exchange assay at | Streptomyces viridifaciens |
| 35 | - |
aminoacylation assay at | Streptomyces viridifaciens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
aminoacylation assay at | Streptomyces viridifaciens |
| 7.5 | - |
ATP-diphosphate exchange assay at | Streptomyces viridifaciens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Streptomyces coelicolor | |
| ATP | - |
Streptomyces avermitilis | |
| ATP | - |
Streptomyces viridifaciens | |
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