Literature summary for 6.1.1.11 extracted from

Godinic, V.; Mocibob, M.; Rocak, S.; Ibba, M.; Weygand-Durasevic, I.
Peroxin Pex21p interacts with the C-terminal noncatalytic domain of yeast seryl-tRNA synthetase and forms a specific ternary complex with tRNA(Ser) (2007), FEBS J., 274, 2788-2799.

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Activating Compound

Activating Compound	Comment	Organism	Structure
Pex21p	the enzyme interacts with the peroxisome biogenesis-related factor Pex21p, the C-terminally appended domain of yeast seryl-tRNA synthetase does not participate in substrate binding, but instead is required for association with Pex21p, Pex21p does not directly bind tRNA, and nor does it possess a tRNA-binding motif, but it instead participates in the formation of a specific ternary complex with seryl-tRNA synthetase and tRNASer, strengthening the interaction of seryl-tRNA synthetase with its cognate tRNASer, overview	Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment)	Organism
expression in strain L40, SerRS mutant enzyme-Pex21p interaction analysis using the yeast two-hybrid system, overview, expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)	Saccharomyces cerevisiae

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of several deletion mutants of seryl-tRNA synthetase, deletion of the 13 C-terminal amino acids abolishes Pex21p binding to seryl-tRNA synthetase, overview	Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	kinetcis	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + L-serine + tRNASer	Saccharomyces cerevisiae	-	AMP + diphosphate + L-seryl-tRNASer	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant mutant enzymes from strain L40 by anion and cation exchange chromatography	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + L-serine + tRNASer	-	Saccharomyces cerevisiae	AMP + diphosphate + L-seryl-tRNASer	-	?
ATP + L-serine + tRNASer	role of Pex21p in enhancing cognate tRNA binding by SerRS	Saccharomyces cerevisiae	AMP + diphosphate + L-seryl-tRNASer	-	?

Synonyms

Synonyms	Comment	Organism
SerRS	-	Saccharomyces cerevisiae
Seryl-tRNA synthetase	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Saccharomyces cerevisiae

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Saccharomyces cerevisiae

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Release 2023.1 (January 2023)