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Literature summary for 6.1.1.10 extracted from

  • Simader, H.; Hothorn, M.; Koehler, C.; Basquin, J.; Simos, G.; Suck, D.
    Structural basis of yeast aminoacyl-tRNA synthetase complex formation revealed by crystal structures of two binary sub-complexes (2006), Nucleic Acids Res., 34, 3968-3979.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 6.1.1.10

Activating Compound

Activating Compound Comment Organism Structure
Arc1p accessory protein Arc1p, SwissProt ID P46672 Saccharomyces cerevisiae
Arc1p-N residues 1-122 of Arc1p, recombinantly expressed, crystal structure analysis, three interacting domains, MetRS-N–Arc1p-N hetero-dimer resembles a classical GST homo-dimer, overview Saccharomyces cerevisiae
Arc1p-N residues 1-122 of Arc1p, recombinantly expressed, crystal structure analysis, three interacting domains, MetRS-N-Arc1p-N hetero-dimer resembles a classical GST homo-dimer, overview Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type and mutant His-tagged MetRSs in Escherichia coli strain BL21(DE3) Saccharomyces cerevisiae

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant MetRS-N-Arc1p-N complex, hanging drop vapour diffusion method, 0.002 ml protein solution containing 15 mg/ml protein in in 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH 7.2 with NaOH, is mixed with 0.002 ml reservoir solution containing 8-14% PEG 20000, 1-3% dioxane, 100 mM bicine, pH 9.0, X-ray diffraction structure determination and analysis at 2.2-2.5 A resolution Saccharomyces cerevisiae
purified recombinant MetRS-N–Arc1p-N complex, hanging drop vapour diffusion method, 0.002 ml protein solution containing 15 mg/ml protein in in 20 mM HEPES, 150 mM NaCl, 5 mM MgCl2, 1 mM DTT, pH 7.2 with NaOH, is mixed with 0.002 ml reservoir solution containing 8–14% PEG 20000, 1–3% dioxane, 100 mM bicine, pH 9.0, X-ray diffraction structure determination and analysis at 2.2-2.5 A resolution Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Saccharomyces cerevisiae 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Saccharomyces cerevisiae
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P00958
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged MetRS from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Saccharomyces cerevisiae AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
additional information MetRS interacts with the accessory protein Arc1p, interaction mode and structure, overview Saccharomyces cerevisiae ?
-
 ?

Subunits

Subunits Comment Organism
More MetRS shows domain swapping Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
methionyl-tRNA synthetase
-
 Saccharomyces cerevisiae
MetRS
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae