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Literature summary for 6.1.1.10 extracted from

  • Senger, B.; Despons, L.; Walter, P.; Jakubowski, H.; Fasiolo, F.
    Yeast cytoplasmic and mitochondrial methionyl-tRNA synthetases: two structural frameworks for identical functions (2001), J. Mol. Biol., 311, 205-216.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of cytoplasmic and mitochondrial isozymes as GST-fusion proteins in the yeast strain RS453, functional complementation of an inactive gene disruption yeast strain by the mitochondrial isozyme located in the cytoplasm Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
A355C site-directed mutagenesis, 115% activity compared to the wild-type enzyme, in vivo complementation of a deficient yeast strain Saccharomyces cerevisiae
C337A/C340A site-directed mutagenesis, inactive Saccharomyces cerevisiae
C350A site-directed mutagenesis, 1.5% activity compared to the wild-type enzyme, no in vivo complementation of a deficient yeast strain Saccharomyces cerevisiae
C350A/C353A site-directed mutagenesis, inactive Saccharomyces cerevisiae
C353A site-directed mutagenesis, catalytically inactive, no in vivo complementation of a deficient yeast strain Saccharomyces cerevisiae
C367A site-directed mutagenesis, catalytically inactive, no in vivo complementation of a deficient yeast strain, mutant shows a second zinc-binding knuckle structure Saccharomyces cerevisiae
D348G site-directed mutagenesis, 4.7% activity compared to the wild-type enzyme, in vivo complementation of a deficient yeast strain Saccharomyces cerevisiae
D370A site-directed mutagenesis, 8.7% activity compared to the wild-type enzyme, in vivo complementation of a deficient yeast strain Saccharomyces cerevisiae
G347R site-directed mutagenesis, catalytically inactive, no in vivo complementation of a deficient yeast strain Saccharomyces cerevisiae
I363N site-directed mutagenesis, 84% activity compared to the wild-type enzyme, in vivo complementation of a deficient yeast strain Saccharomyces cerevisiae
additional information construction of an inactive strain by gene disruption Saccharomyces cerevisiae
P338I site-directed mutagenesis, 74% activity compared to the wild-type enzyme, in vivo complementation of a deficient yeast strain Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline complete inhibition of the cytoplasmic isozyme at 1 mM Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0004
-
tRNAMet recombinant mitochondrial isozyme as GST-fusion protein, pH 7.8, 25°C Saccharomyces cerevisiae
0.009
-
tRNAMet recombinant cytoplasmic isozyme, pH 7.8, 25°C Saccharomyces cerevisiae
0.015
-
tRNAMet recombinant cytoplasmic isozyme as GST-fusion protein, pH 7.8, 25°C Saccharomyces cerevisiae
1.2
-
CoA methionylation reaction, cytoplasmic isozyme, pH 7.8, 25°C Saccharomyces cerevisiae
1.8
-
L-homocysteine editing reaction, mitochondrial isozyme, pH 7.8, 25°C Saccharomyces cerevisiae
3.1
-
L-homocysteine editing reaction, cytoplasmic isozyme, pH 7.8, 25°C Saccharomyces cerevisiae
6.3
-
CoA methionylation reaction, mitochondrial isozyme, pH 7.8, 25°C Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm cytoplasmic isozyme has a zinc-finger motif and an insertion domain that divides the nucleotide-binding fold into 2 halves Saccharomyces cerevisiae 5737
-
mitochondrion mitochondrial isozyme contains no zinc-finger motif and no insertion domain in the nucleotide-binding fold Saccharomyces cerevisiae 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ 2 binding sites, 0.14 mM tightly bound zinc is present in 1 mol of cytoplasmic isozyme, but not in the mitochondrial one, required for stability and activity of the cytoplasmic isozyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-homocysteine + tRNAMet Saccharomyces cerevisiae edition and aminoacylation by cytoplasmic and mitochondrial isozyme ?
-
?
ATP + L-methionine + tRNAMet Saccharomyces cerevisiae initiator and elongation tRNAMet, cytoplasmic and mitochondrial isozyme AMP + diphosphate + L-methionyl-tRNAMet
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
2 cytoplasmic and mitochondrial isozymes
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion protein of the mitochondrial isozyme Saccharomyces cerevisiae

Reaction

Reaction Comment Organism Reaction ID
ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet amino acid residues D138, R139, and F135 are important for activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-homocysteine + tRNAMet edition and aminoacylation by cytoplasmic and mitochondrial isozyme Saccharomyces cerevisiae ?
-
?
ATP + L-methionine + tRNAMet initiator and elongation tRNAMet, cytoplasmic and mitochondrial isozyme Saccharomyces cerevisiae AMP + diphosphate + L-methionyl-tRNAMet
-
?
CoA + L-methionine
-
Saccharomyces cerevisiae Met-S-CoA
-
?
additional information the isozymes also perform the ATP-diphosphate exchange reaction Saccharomyces cerevisiae ?
-
?

Synonyms

Synonyms Comment Organism
Methionine translase
-
Saccharomyces cerevisiae
Methionine--tRNA ligase
-
Saccharomyces cerevisiae
Methionyl tRNA synthetase
-
Saccharomyces cerevisiae
Methionyl-transfer ribonucleate synthetase
-
Saccharomyces cerevisiae
Methionyl-transfer ribonucleic acid synthetase
-
Saccharomyces cerevisiae
Methionyl-transfer RNA synthetase
-
Saccharomyces cerevisiae
MetRS
-
Saccharomyces cerevisiae
Synthetase, methionyl-transfer ribonucleate
-
Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
tRNAMet recombinant mitochondrial isozyme as GST-fusion protein, pH 7.8, 25°C Saccharomyces cerevisiae
0.5
-
L-homocysteine editing reaction, mitochondrial isozyme, pH 7.8, 25°C Saccharomyces cerevisiae
0.69
-
L-homocysteine editing reaction, cytoplasmic isozyme, pH 7.8, 25°C Saccharomyces cerevisiae
0.7
-
CoA methionylation reaction, cytoplasmic isozyme, pH 7.8, 25°C Saccharomyces cerevisiae
1
-
tRNAMet recombinant cytoplasmic isozyme as GST-fusion protein, pH 7.8, 25°C Saccharomyces cerevisiae
1.3
-
tRNAMet recombinant cytoplasmic isozyme, pH 7.8, 25°C Saccharomyces cerevisiae
1.6
-
CoA methionylation reaction, mitochondrial isozyme, pH 7.8, 25°C Saccharomyces cerevisiae
6.08
-
L-homocysteine editing reaction, cytoplasmic isozyme, pH 7.8, 25°C Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
Saccharomyces cerevisiae