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Literature summary for 6.1.1.10 extracted from

  • Jo, Y.J.; Lee, S.W.; Jo, M.K.; Lee, J.W.; Kang, M.K.; Yoon, J.H.; Kim, S.
    Methionine analogue probes functionally important residues in active site of methionyl-tRNA synthetase (1999), J. Biochem. Mol. Biol., 32, 547-553.
No PubMed abstract available
Search for more literature for 6.1.1.10

Protein Variants

Protein Variants Comment Organism
T10M natural mutant, 5% activity compared to the wild-type, complementation of an enzyme-deficient Escherichia coli strain, no inhibition by L-methionine hydroxamate Escherichia coli
Y15A natural mutant, very low residual activity, complementation of an enzyme-deficient Escherichia coli strain Escherichia coli
Y94H natural mutant, unstable, no complementation of an enzyme-deficient Escherichia coli strain Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
L-methionine hydroxamate substrate analogue, inhibition mechanism, no inhibition of mutant T10M Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.9
-
 ATP wild-type, pH 7.5, 37°C Escherichia coli
7.9
-
 L-methionine wild-type, pH 7.5, 37°C Escherichia coli
58
-
 ATP mutant T10M, pH 7.5, 37°C Escherichia coli
58
-
 L-methionine mutant T10M, pH 7.5, 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + L-methionine + tRNAMet Escherichia coli
-
 AMP + diphosphate + L-methionyl-tRNAMet
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Reaction

Reaction Comment Organism Reaction ID
ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet active site structure and reaction mechanism involving Tyr15 and Thr10, Tyr94 is involved in maintaining the native enzyme conformation Escherichia coli
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
 Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
ATP + L-methionine + tRNAMet formation of an methionine adenylate reaction intermediate Escherichia coli AMP + diphosphate + L-methionyl-tRNAMet
-
 ?
additional information enzyme also performs the ATP-diphosphate exchange reaction Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
Methionine translase
-
 Escherichia coli
Methionine--tRNA ligase
-
 Escherichia coli
Methionyl tRNA synthetase
-
 Escherichia coli
Methionyl-transfer ribonucleate synthetase
-
 Escherichia coli
Methionyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
Methionyl-transfer RNA synthetase
-
 Escherichia coli
MetRS
-
 Escherichia coli
Synthetase, methionyl-transfer ribonucleate
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4
-
 ATP mutant T10M, pH 7.5, 37°C Escherichia coli
3.4
-
 L-methionine mutant T10M, pH 7.5, 37°C Escherichia coli
5.6
-
 ATP wild-type, pH 7.5, 37°C Escherichia coli
5.6
-
 L-methionine wild-type, pH 7.5, 37°C Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0196
-
 L-methionine hydroxamate pH 8.0 Escherichia coli