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Literature summary for 6.1.1.10 extracted from
- Structure and function of the C-terminal domain of methionyl-tRNA synthetase (2002), Biochemistry, 41, 13003-13011.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 12 kDa protein 2 | the recombinant 12 kDa protein 2 plays an important role in dimerization of the enzyme | Pyrococcus abyssi |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of mutant enzymes | Escherichia coli |
| gene metS, expression in Escherichia coli leads to production of 2 thermostable proteins | Pyrococcus abyssi |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| recombinant 12 kDa protein 2, 20% PEG 8000, cryoprotection in 10% ethylene glycol, 50 mM KH2PO4, X-ray diffraction structure determination at 2.0 A, and analysis | Pyrococcus abyssi |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D666A | activity is similar to the wild-type enzyme | Escherichia coli |
| additional information | construction of C-terminal truncated mutant, removal of beta10 strand and insertion of a stop codon at position 666, M665 | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0044 | - |
tRNAMet | aminoacylation reaction, wild-type | Escherichia coli |  |
| 0.0058 | - |
tRNAMet | aminoacylation reaction, C-terminally truncated mutant | Escherichia coli | |
| 0.0064 | - |
tRNAMet | aminoacylation reaction, mutant D666A | Escherichia coli | |
| 0.0087 | - |
tRNAMet | aminoacylation reaction, mutant M665 | Escherichia coli | |
| 0.02 | - |
tRNAMet | ATP-diphosphate exchange reaction, wild-type | Escherichia coli | |
| 0.022 | - |
tRNAMet | ATP-diphosphate exchange reaction, C-terminally truncated mutant | Escherichia coli | |
| 0.029 | - |
tRNAMet | ATP-diphosphate exchange reaction, mutant D666A | Escherichia coli | |
| 0.034 | - |
tRNAMet | ATP-diphosphate exchange reaction, mutant M665 | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 12000 | - |
2 * 12000, recombinant protein 2 expressed in Escherichia coli, SDS-PAGE | Pyrococcus abyssi |
| 24000 | - |
recombinant 12 kDa protein 2, gel filtration | Pyrococcus abyssi |
| 84000 | - |
x * 84000, recombinant protein 1 expressed in Escherichia coli, SDS-PAGE | Pyrococcus abyssi |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | Escherichia coli | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
| ATP + L-methionine + tRNAMet | Pyrococcus abyssi | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Pyrococcus abyssi | Q9V011 | - |
- |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet | amino acid residues involved in tRNA binding are K30, N66, S78, and M81 | Pyrococcus abyssi |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + L-methionine + tRNAMet | - |
Escherichia coli | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
| ATP + L-methionine + tRNAMet | - |
Pyrococcus abyssi | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
| ATP + L-methionine + tRNAMet | dimerization of the enzyme is required for affinity to tRNAMet | Pyrococcus abyssi | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
| additional information | the enzyme also performs the ATP-diphosphate exchange reaction | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 84000, recombinant protein 1 expressed in Escherichia coli, SDS-PAGE | Pyrococcus abyssi |
| dimer | 2 * 12000, recombinant protein 2 expressed in Escherichia coli, SDS-PAGE | Pyrococcus abyssi |
| monomer | mutant D666A ia monomeric, M665 mutant is monomeic in absence of NgCl2, and dimeric in presence of 10 mM MgCl2, native PAGE | Escherichia coli |
| More | dimerization of the enzyme is required for affinity to tRNAMet | Pyrococcus abyssi |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Methionine translase | - |
Escherichia coli |
| Methionine translase | - |
Pyrococcus abyssi |
| Methionine--tRNA ligase | - |
Escherichia coli |
| Methionine--tRNA ligase | - |
Pyrococcus abyssi |
| Methionyl tRNA synthetase | - |
Escherichia coli |
| Methionyl tRNA synthetase | - |
Pyrococcus abyssi |
| Methionyl-transfer ribonucleate synthetase | - |
Escherichia coli |
| Methionyl-transfer ribonucleate synthetase | - |
Pyrococcus abyssi |
| Methionyl-transfer ribonucleic acid synthetase | - |
Escherichia coli |
| Methionyl-transfer ribonucleic acid synthetase | - |
Pyrococcus abyssi |
| Methionyl-transfer RNA synthetase | - |
Escherichia coli |
| Methionyl-transfer RNA synthetase | - |
Pyrococcus abyssi |
| MetRS | - |
Escherichia coli |
| MetRS | - |
Pyrococcus abyssi |
| Synthetase, methionyl-transfer ribonucleate | - |
Escherichia coli |
| Synthetase, methionyl-transfer ribonucleate | - |
Pyrococcus abyssi |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 51 | - |
wild-type enzyme, half-life 16 min | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | 8 | tRNAMet | ATP-diphosphate exchange reaction, mutant M665 | Escherichia coli | |
| 2.8 | - |
tRNAMet | aminoacylation reaction, wild-type | Escherichia coli | |
| 3.1 | - |
tRNAMet | aminoacylation reaction, mutant D666A | Escherichia coli | |
| 3.6 | - |
tRNAMet | aminoacylation reaction, C-terminally truncated mutant | Escherichia coli | |
| 4.4 | - |
tRNAMet | aminoacylation reaction, mutant M665 | Escherichia coli | |
| 23 | - |
tRNAMet | ATP-diphosphate exchange reaction, mutant D666A | Escherichia coli | |
| 42 | - |
tRNAMet | ATP-diphosphate exchange reaction, wild-type | Escherichia coli | |
| 47 | - |
tRNAMet | ATP-diphosphate exchange reaction, C-terminally truncated mutant | Escherichia coli | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
| ATP | - |
Pyrococcus abyssi | |
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