Activating Compound | Comment | Organism | Structure |
---|---|---|---|
elongation factor EF-1alpha | activates, assists in dissociation of Met-tRNAMet from the enzyme, the aminoacylated tRNA is transfered from to the enzyme to the elongation factor | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of the wild-type, and exchange mutant enzymes in Escherichia coli BL21(DE3), cloning and expression of the catalytic domain, amino acid residues A215-K823, and the C-terminal extension in Escherichia coli BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K860A | site-directed mutagenesis, reduced activity | Homo sapiens |
K863A | site-directed mutagenesis, reduced activity | Homo sapiens |
K866A | site-directed mutagenesis, reduced activity | Homo sapiens |
K880A | site-directed mutagenesis, altered kinetics | Homo sapiens |
R857A | site-directed mutagenesis, reduced activity | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | dissociation constants for wild-type and mutant enzymes | Homo sapiens | |
0.0022 | - |
tRNAMet | C-terminal extension, pH 7.5, 25°C | Homo sapiens | |
0.0033 | - |
tRNAMet | mutant K863A, pH 7.5, 25°C | Homo sapiens | |
0.0035 | - |
tRNAMet | wild-type enzyme, pH 7.5, 25°C | Homo sapiens | |
0.0039 | - |
tRNAMet | wild-type enzyme in the multi-enzyme complex and mutant K866A, pH 7.5, 25°C | Homo sapiens | |
0.0057 | - |
tRNAMet | mutant R857A, pH 7.5, 25°C | Homo sapiens | |
0.0163 | - |
tRNAMet | mutant K880A, pH 7.5, 25°C | Homo sapiens | |
0.0172 | - |
tRNAMet | mutant K860A, pH 7.5, 25°C | Homo sapiens | |
0.032 | - |
tRNAMet | catalytic domain, pH 7.5, 25°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | Homo sapiens | - |
AMP + diphosphate + L-methionyl-tRNAMet | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type enzyme, mutants and fragments from Escherichia coli | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + L-methionine + tRNAMet = AMP + diphosphate + L-methionyl-tRNAMet | mechanism, catalytic domain is formed by amino acid residues A215-K823 | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Homo sapiens | AMP + diphosphate + L-methionyl-tRNAMet | - |
r | |
ATP + L-methionine + tRNAMet | the C-terminal ancillary RNA-binding domain is important for activity and has dual function provided by 2 structural motifs: 1. the helix-turn-helix HTH motif, which confers rate-limiting dissociation of the aminoaclyted tRNA from the enzyme, and 2. the KGKKKK lysine-rich cluster LRC, which is probably involved in accelerating the association step of deacylated tRNA | Homo sapiens | AMP + diphosphate + L-methionyl-tRNAMet | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Methionine translase | - |
Homo sapiens |
Methionine--tRNA ligase | - |
Homo sapiens |
Methionyl tRNA synthetase | - |
Homo sapiens |
Methionyl-transfer ribonucleate synthetase | - |
Homo sapiens |
Methionyl-transfer ribonucleic acid synthetase | - |
Homo sapiens |
Methionyl-transfer RNA synthetase | - |
Homo sapiens |
MetRS | - |
Homo sapiens |
Synthetase, methionyl-transfer ribonucleate | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.09 | - |
tRNAMet | C-terminal extension, pH 7.5, 25°C | Homo sapiens | |
0.15 | - |
tRNAMet | wild-type enzyme, pH 7.5, 25°C | Homo sapiens | |
0.22 | - |
tRNAMet | mutant K863A, pH 7.5, 25°C | Homo sapiens | |
0.23 | - |
tRNAMet | mutant K866A, pH 7.5, 25°C | Homo sapiens | |
0.46 | - |
tRNAMet | wild-type enzyme in the multi-enzyme complex, pH 7.5, 25°C | Homo sapiens | |
0.47 | - |
tRNAMet | mutant R857A, pH 7.5, 25°C | Homo sapiens | |
0.85 | - |
tRNAMet | mutant K860A, pH 7.5, 25°C | Homo sapiens | |
1.03 | - |
tRNAMet | mutant K880A, pH 7.5, 25°C | Homo sapiens | |
2.4 | - |
tRNAMet | catalytic domain, pH 7.5, 25°C | Homo sapiens | |
6.08 | - |
tRNAMet | mutant K880A, pH 7.5, 25°C | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |