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Literature summary for 6.1.1.10 extracted from

  • Kellermann, O.; Viel, C.; Waller, J.P.
    Methionyl-tRNA synthetase from sheep mammary gland. Purification of a fully active monomeric enzyme derived from high-molecular-weight complexes by controlled proteolysis (1978), Eur. J. Biochem., 88, 197-204.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
1 * 78000, SDS-PAGE, fully active monomeric enzyme derived from high-molecular-weight complexes by controlled proteolysis Ovis aries

Organism

Organism UniProt Comment Textmining
Ovis aries
-
-
-

Purification (Commentary)

Purification (Comment) Organism
fully active monomeric enzyme derived from high-molecular-weight complexes by controlled proteolysis, trypsin-treatment Ovis aries

Source Tissue

Source Tissue Comment Organism Textmining
mammary gland
-
Ovis aries
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.405
-
-
Ovis aries

Storage Stability

Storage Stability Organism
-20°C, potassium phosphate buffer, pH 7.5, 50% glycerol, 1 mM dithioerythritol, stable for at least 6 months Ovis aries

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + L-methionine + tRNAMet
-
Ovis aries AMP + diphosphate + L-methionyl-tRNAMet
-
?

Subunits

Subunits Comment Organism
monomer 1 * 78000, SDS-PAGE, fully active monomeric enzyme derived from high-molecular-weight complexes by controlled proteolysis Ovis aries