Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cysteic acid | stimulates | Paracoccus denitrificans | |
cysteine | stimulates | Paracoccus denitrificans | |
SO32- | stimulates | Paracoccus denitrificans |
General Stability | Organism |
---|---|
stability is increased by presence of a reducing agent. In the presence of 5 mM DTT purified enzyme is stable for at least 8 months at -15°C | Paracoccus denitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3'-AMP | - |
Paracoccus denitrificans | |
5'-AMP | - |
Paracoccus denitrificans | |
ADP | - |
Paracoccus denitrificans | |
ATP | in free form | Paracoccus denitrificans | |
D-methionine | - |
Paracoccus denitrificans | |
EDTA | 10 mM, weak | Paracoccus denitrificans | |
glutathione | - |
Paracoccus denitrificans | |
iodoacetamide | 10 mM, weak | Paracoccus denitrificans | |
Mg2+ | in free form | Paracoccus denitrificans | |
NaF | 10 mM, weak | Paracoccus denitrificans | |
NEM | - |
Paracoccus denitrificans | |
O-acetylserine | - |
Paracoccus denitrificans | |
p-chloromercuribenzoate | - |
Paracoccus denitrificans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
L-Met | - |
Paracoccus denitrificans | |
0.31 | - |
selenomethionine | - |
Paracoccus denitrificans | |
0.383 | - |
norleucine | - |
Paracoccus denitrificans | |
0.87 | - |
ATP | - |
Paracoccus denitrificans | |
28.6 | - |
norleucine | - |
Paracoccus denitrificans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | supports methionine-dependent ATP-diphosphate exchange with 14% of the efficiency of Mg2+ | Paracoccus denitrificans | |
Co2+ | supports methionine-dependent ATP-diphosphate exchange with 20% of the efficiency of Mg2+ | Paracoccus denitrificans | |
Mg2+ | absolutely dependent on | Paracoccus denitrificans | |
Mg2+ | 2 | Paracoccus denitrificans | |
Mn2+ | supports methionine-dependent ATP-diphosphate exchange with 15% of the efficiency of Mg2+ | Paracoccus denitrificans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
- |
- |
Paracoccus denitrificans 8944 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Paracoccus denitrificans |
Storage Stability | Organism |
---|---|
-15°C, 5 mM DTT, purified enzyme stable for at least 8 months | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Paracoccus denitrificans | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
ATP + L-methionine + tRNAMet | - |
Paracoccus denitrificans 8944 | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
ATP + L-norleucine + tRNAMet | - |
Paracoccus denitrificans | AMP + diphosphate + L-norleucyl-tRNAMet | - |
? | |
ATP + methionine + hydroxylamine | - |
Paracoccus denitrificans | methionine hydroxamate + AMP + diphosphate | - |
? | |
ATP + methionine + hydroxylamine | - |
Paracoccus denitrificans 8944 | methionine hydroxamate + AMP + diphosphate | - |
? | |
ATP + selenomethionine + tRNAMet | - |
Paracoccus denitrificans | AMP + diphosphate + selenomethionyl-tRNAMet | - |
? | |
ATP + selenomethionine + tRNAMet | - |
Paracoccus denitrificans 8944 | AMP + diphosphate + selenomethionyl-tRNAMet | - |
? | |
additional information | methionine-dependent ATP-diphosphate exchange | Paracoccus denitrificans | ? | - |
? | |
additional information | also catalyzes L-homocysteine, L-selenocysteine and norleucine-dependent ATP-diphosphate exchange | Paracoccus denitrificans | ? | - |
? | |
additional information | methionine-dependent ATP-diphosphate exchange | Paracoccus denitrificans 8944 | ? | - |
? | |
additional information | also catalyzes L-homocysteine, L-selenocysteine and norleucine-dependent ATP-diphosphate exchange | Paracoccus denitrificans 8944 | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Paracoccus denitrificans |
8 | 8.5 | - |
Paracoccus denitrificans |