KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.133 | - |
L-Met | ATP-diphosphate exchange, enzyme form B | Mycolicibacterium smegmatis | |
0.166 | - |
L-Met | ATP-diphosphate exchange, enzyme form A | Mycolicibacterium smegmatis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolutely dependent on | Mycolicibacterium smegmatis | |
Mg2+ | optimal concentration: 10 mM | Mycolicibacterium smegmatis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
10000 | - |
2 * 31000 + 2 * 10000, enzyme form B, SDS-PAGE | Mycolicibacterium smegmatis |
31000 | - |
2 * 31000, enzyme form A, SDS-PAGE | Mycolicibacterium smegmatis |
31000 | - |
2 * 31000 + 2 * 10000, enzyme form B, SDS-PAGE | Mycolicibacterium smegmatis |
65000 | - |
enzyme form A, gel filtration | Mycolicibacterium smegmatis |
85000 | - |
enzyme form B, gel filtration | Mycolicibacterium smegmatis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
two enzyme forms: A and B | Mycolicibacterium smegmatis |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
enzyme form B | Mycolicibacterium smegmatis |
0.09 | - |
enzyme form A | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Mycolicibacterium smegmatis | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
additional information | no other amino acid than methionine is activated in ATP-diphosphate exchange | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | enzyme forms A and B show differences in their capacity to recognize the cognate tRNAs | Mycolicibacterium smegmatis | ? | - |
? | |
additional information | methionine-dependent ATP-diphosphate exchange | Mycolicibacterium smegmatis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 31000, enzyme form A, SDS-PAGE | Mycolicibacterium smegmatis |
tetramer | 2 * 31000 + 2 * 10000, enzyme form B, SDS-PAGE | Mycolicibacterium smegmatis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Mycolicibacterium smegmatis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.94 | - |
L-Met | ATP-diphosphate exchange, enzyme form A | Mycolicibacterium smegmatis | |
3.13 | - |
L-Met | ATP-diphosphate exchange, enzyme form B | Mycolicibacterium smegmatis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
sodium cacodylate or Tris buffer | Mycolicibacterium smegmatis |