General Stability | Organism |
---|---|
Mg2+, required for stability, enzyme loses 80% of its activity when stored at -20°C for 3 days in buffer lacking Mg2+ | Triticum aestivum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | in presence of 15 mM Mg2+, K+ stimulates up to concentration of about 50 mM, inhibits at higher concentrations. Unable to stimulate aminoacylation in absence of Mg2+ | Triticum aestivum | |
Mg2+ | 2 (ATP-diphosphate exchange), 1.5 (aminoacylation) | Triticum aestivum | |
Mn2+ | optimal Mn2+/ATP ratio: 1 (ATP-diphosphate exchange), 1.5 (aminoacylation) | Triticum aestivum | |
NH4+ | in presence of 15 mM Mg2+, NH4+ stimulates up to a concentration of about 50 mM, inhibits at higher concentrations. In presence of 0.5 mM Mg2+, NH4+ alone stimulates aminoacylation | Triticum aestivum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
2 * 74000, SDS-PAGE | Triticum aestivum |
165000 | - |
gel filtration | Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Triticum aestivum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
germ | - |
Triticum aestivum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.087 | - |
- |
Triticum aestivum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-methionine + tRNAMet | - |
Triticum aestivum | AMP + diphosphate + L-methionyl-tRNAMet | - |
? | |
additional information | methionine-dependent ATP-diphosphate exchange | Triticum aestivum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 74000, SDS-PAGE | Triticum aestivum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.9 | ATP-diphosphate exchange | Triticum aestivum |
8.1 | 8.4 | aminoacylation | Triticum aestivum |