Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells | Aeropyrum pernix |
expression in Escherichia coli | Aeropyrum pernix |
Protein Variants | Comment | Organism |
---|---|---|
D172H | mutant enzyme shows a significant reductions in tyrosylation activity | Aeropyrum pernix |
D172N | mutant enzyme shows a significant reductions in tyrosylation activity | Aeropyrum pernix |
D172P | mutant enzyme shows a significant reductions in tyrosylation activity | Aeropyrum pernix |
D172P | the mutation completely abolishes tyrosylation activity | Aeropyrum pernix |
additional information | Asp172 mutants shows completely abolished tyrosylation activity, whereas mutation at Tyr39 has no effect on activity | Aeropyrum pernix |
Y39E | mutant enzyme maintains tyrosylation activity | Aeropyrum pernix |
Y39G | mutant enzyme maintains tyrosylation activity. Although the wild-type enzyme shows specific tyrosylation activity but not aminoacylation activity for 4-azido-L-phenylalanine, the Y39G mutant exhibits near identical aminoacylation activity of both tyrosine and 4-azido-L-phenylalanine | Aeropyrum pernix |
Y39G | the mutant exhibits near identical aminoacylation activity of both L-tyrosine and 4-azide-L-phenylalanine | Aeropyrum pernix |
Y39G/D172P | reduction in tyrosylation activity, the mutant shows specific aminoacylation activity for 4-azide-L-phenylalanine | Aeropyrum pernix |
Y39G/D172P | the double mutant shows a reduction in tyrosylation activity | Aeropyrum pernix |
Y39K | mutant enzyme maintains tyrosylation activity | Aeropyrum pernix |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters for anticodon mutants of tRNATyr and for for acceptor stem mutants of tRNATyr | Aeropyrum pernix | |
0.00022 | - |
tRNATyr | 55°C, pH 8.0 | Aeropyrum pernix | |
0.00022 | - |
tRNATyr | wild type enzyme, at 55°C, in 100 mM HEPES-NaOH (pH 8.0), 10 mM MgCl2, 10 mM KCl | Aeropyrum pernix |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-tyrosine + tRNATyr | Aeropyrum pernix | - |
AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
ATP + L-tyrosine + tRNATyr | Aeropyrum pernix DSM 11879 | - |
AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aeropyrum pernix | - |
- |
- |
Aeropyrum pernix | Q9YA64 | - |
- |
Aeropyrum pernix DSM 11879 | Q9YA64 | - |
- |
Purification (Comment) | Organism |
---|---|
Cibacron Blue 3GA column chromatography | Aeropyrum pernix |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-(5-hydroxypyridin-2-yl)-L-alanine + tRNATyr | - |
Aeropyrum pernix | ? | - |
? | |
ATP + L-tyrosine + tRNATyr | - |
Aeropyrum pernix | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
ATP + L-tyrosine + tRNATyr | - |
Aeropyrum pernix DSM 11879 | AMP + diphosphate + L-tyrosyl-tRNATyr | - |
? | |
additional information | no activity with 4-azido-L-phenylalanine, 4-amino-L-phenylalanine, 4-nitro-L-phenylalanine, 4-methyl-L-phenylalanine, 4-fluoro-L-phenylalanine, 4-chloro-L-phenylalanine, 4-bromo-L-phenylalanine | Aeropyrum pernix | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Tyrosyl-tRNA synthetase | - |
Aeropyrum pernix |
TyrRS | - |
Aeropyrum pernix |
TyrRSs | - |
Aeropyrum pernix |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
assay at | Aeropyrum pernix |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters for anticodon mutants of tRNATyr and for for acceptor stem mutants of tRNATyr | Aeropyrum pernix | |
0.29 | - |
tRNATyr | 55°C, pH 8.0 | Aeropyrum pernix | |
0.29 | - |
tRNATyr | wild type enzyme, at 55°C, in 100 mM HEPES-NaOH (pH 8.0), 10 mM MgCl2, 10 mM KCl | Aeropyrum pernix |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Aeropyrum pernix |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Aeropyrum pernix |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters for anticodon mutants of tRNATyr and for for acceptor stem mutants of tRNATyr | Aeropyrum pernix | |
1300 | - |
tRNATyr | wild type enzyme, at 55°C, in 100 mM HEPES-NaOH (pH 8.0), 10 mM MgCl2, 10 mM KCl | Aeropyrum pernix | |
1318 | - |
tRNATyr | 55°C, pH 8.0 | Aeropyrum pernix |