Any feedback?
Literature summary for 6.1.1.1 extracted from
- Chloride affects the interaction between tyrosyl-tRNA synthetase and tRNA (1999), Biochim. Biophys. Acta, 1472, 51-61.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | inhibition is weakened by chloride | Escherichia coli |  |
| AMP | inhibition is weakened by chloride | Geobacillus stearothermophilus | |
| chloride | inhibition in presence of 1 mM free Mg2+, no inhibition in presence of 10 mM free Mg2+ | Escherichia coli | |
| chloride | inhibition in presence of 1 mM free Mg2+, no inhibition in presence of 10 mM free Mg2+ | Geobacillus stearothermophilus | |
| diphosphate | inhibition is strengthened by chloride | Escherichia coli | |
| diphosphate | inhibition is strengthened by chloride | Geobacillus stearothermophilus | |
| additional information | no inhibition by acetate up to 200 mM in presence of 1 mM free Mg2+ | Escherichia coli | |
| sulfate | inhibition in presence of 1 mM free Mg2+ | Escherichia coli | |
| sulfate | inhibition in presence of 1 mM free Mg2+ | Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Escherichia coli | |
| additional information | - |
additional information | kinetics | Geobacillus stearothermophilus | |
| 0.000022 | - |
tRNATyr | in absence of KCl, pH 7.4, 30°C | Escherichia coli | |
| 0.000037 | - |
tRNATyr | in presence of 50 mM KCl, pH 7.4, 30°C | Escherichia coli | |
| 0.000093 | - |
tRNATyr | in presence of 100 mM KCl, pH 7.4, 30°C | Escherichia coli | |
| 0.00024 | - |
tRNATyr | in presence of 150 mM KCl, pH 7.4, 30°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required, shows regulatory function | Geobacillus stearothermophilus | |
| Mg2+ | required, shows regulatory function, binding of 1 Mg2+ per tRNA molecule for the transfer reaction, binding to the tRNA is weakened by chloride | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + tyrosine + tRNATyr | Escherichia coli | - |
AMP + Tyr-tRNATyr + diphosphate | - |
? | |
| ATP + tyrosine + tRNATyr | Geobacillus stearothermophilus | - |
AMP + Tyr-tRNATyr + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
C6, purified enzyme | - |
| Geobacillus stearothermophilus | - |
strain ATCC 12980 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| ATP + L-tyrosine + tRNATyr = AMP + diphosphate + L-tyrosyl-tRNATyr | mechanism | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.8 | - |
purified enzyme | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + tyrosine + tRNATyr | - |
Escherichia coli | AMP + Tyr-tRNATyr + diphosphate | - |
? | |
| ATP + tyrosine + tRNATyr | - |
Geobacillus stearothermophilus | AMP + Tyr-tRNATyr + diphosphate | - |
? | |
| ATP + tyrosine + tRNATyr | tRNATyr substrate from Escherichia coli | Geobacillus stearothermophilus | AMP + Tyr-tRNATyr + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Tyrosine tRNA synthetase | - |
Escherichia coli |
| Tyrosyl-tRNA synthetase | - |
Geobacillus stearothermophilus |
| TyrRS | - |
Escherichia coli |
| TyrRS | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Escherichia coli |
| 30 | - |
assay at | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Escherichia coli |
| 7.4 | - |
assay at | Geobacillus stearothermophilus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Escherichia coli | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | inhibition kinetics | Escherichia coli | |
| additional information | - |
additional information | inhibition kinetics | Geobacillus stearothermophilus | |
| 0.048 | - |
diphosphate | in presence of 150 mM KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
| 0.056 | - |
diphosphate | in presence of 0.9 mM KCl, pH 7.4, 30°C | Escherichia coli | |
| 0.1 | - |
diphosphate | in presence of 50 mM KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
| 0.12 | - |
diphosphate | in presence of 0.5 mM KCl, pH 7.4, 30°C | Escherichia coli | |
| 0.14 | - |
diphosphate | in absence of KCl, pH 7.4, 30°C | Escherichia coli | |
| 0.23 | - |
diphosphate | in absence of KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
| 0.32 | - |
AMP | in absence of KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
| 0.35 | - |
AMP | in absence of KCl, pH 7.4, 30°C | Escherichia coli | |
| 0.5 | - |
AMP | in presence of 0.5 mM KCl, pH 7.4, 30°C | Escherichia coli | |
| 0.5 | - |
AMP | in presence of 50 mM KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
| 0.9 | - |
AMP | in presence of 0.9 mM KCl, pH 7.4, 30°C | Escherichia coli | |
| 2.15 | - |
AMP | in presence of 150 mM KCl, and in presence of 1 mM free Mg2+, pH 7.4, 30°C | Geobacillus stearothermophilus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
