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Literature summary for 5.6.2.6 extracted from

  • Despins, S.; Issur, M.; Bougie, I.; Bisaillon, M.
    Deciphering the molecular basis for nucleotide selection by the West Nile virus RNA helicase (2010), Nucleic Acids Res., 38, 5493-5506.
    View publication on PubMed
Search for more literature for 5.6.2.6

Protein Variants

Protein Variants Comment Organism
D172A the ration of (kcat/Km)ATP/(kcat/Km)GTP is 41% of the ratio determined for the wild-type enzyme West Nile virus
E169A the ration of (kcat/Km)ATP/(kcat/Km)GTP is 38% of the ratio determined for the wild-type enzyme West Nile virus
E173A the ration of (kcat/Km)ATP/(kcat/Km)GTP is 17% of the ratio determined for the wild-type enzyme West Nile virus
E180A the ration of (kcat/Km)ATP/(kcat/Km)GTP is 35% of the ratio determined for the wild-type enzyme West Nile virus
E182A the ration of (kcat/Km)ATP/(kcat/Km)GTP is 29% of the ratio determined for the wild-type enzyme West Nile virus
F179A the ration of (kcat/Km)ATP/(kcat/Km)GTP is 19% of the ratio determined for the wild-type enzyme West Nile virus
K186A the ration of (kcat/Km)ATP/(kcat/Km)GTP is 15% of the ratio determined for the wild-type enzyme West Nile virus
K187A the ration of (kcat/Km)ATP/(kcat/Km)GTP is 8% of the ratio determined for the wild-type enzyme West Nile virus
Q188A the ration of (kcat/Km)ATP/(kcat/Km)GTP is 33% of the ratio determined for the wild-type enzyme West Nile virus
R170A the ration of (kcat/Km)ATP/(kcat/Km)GTP is 31% of the ratio determined for the wild-type enzyme West Nile virus
R185A inactive mutant enzyme West Nile virus

Inhibitors

Inhibitors Comment Organism Structure
2',3'-dideoxy-ATP
-
 West Nile virus
2',3'-dideoxy-GTP
-
 West Nile virus
2'-deoxy-ATP
-
 West Nile virus
2'-deoxy-GTP
-
 West Nile virus
2'-deoxy-L-GTP
-
 West Nile virus
2'-fluoro-2'-deoxy-ATP
-
 West Nile virus
2-amino-ATP
-
 West Nile virus
2-hydroxy-ATP
-
 West Nile virus
3'-deoxy-ATP
-
 West Nile virus
6-methyl-thio-ITP
-
 West Nile virus
6-thio-GTP
-
 West Nile virus
7-methyl-GTP
-
 West Nile virus
8-bromo-ATP
-
 West Nile virus
ara-ATP
-
 West Nile virus
ATP
-
 West Nile virus
GTP
-
 West Nile virus
ITP
-
 West Nile virus
N1-methyl-ATP
-
 West Nile virus
N1-methyl-GTP
-
 West Nile virus
N6-methyl-ATP
-
 West Nile virus
ribavirin triphosphate
-
 West Nile virus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.09
-
 GTP pH 7.5, 37°C, mutant enzyme F179A West Nile virus
0.09
-
 GTP pH 7.5, 37°C, mutant enzyme K187A West Nile virus
0.1
-
 GTP pH 7.5, 37°C, mutant enzyme K186A West Nile virus
0.11
-
 GTP pH 7.5, 37°C, mutant enzyme E173A West Nile virus
0.14
-
 ATP pH 7.5, 37°C, wild-type enzyme West Nile virus
0.18
-
 ATP pH 7.5, 37°C, mutant enzyme D172A West Nile virus
0.18
-
 ATP pH 7.5, 37°C, mutant enzyme F179A West Nile virus
0.2
-
 ATP pH 7.5, 37°C, mutant enzyme R170A West Nile virus
0.2
-
 ATP pH 7.5, 37°C, mutant enzyme E173A West Nile virus
0.22
-
 ATP pH 7.5, 37°C, mutant enzyme E169A West Nile virus
0.23
-
 GTP pH 7.5, 37°C, mutant enzyme E182A West Nile virus
0.24
-
 ATP pH 7.5, 37°C, mutant enzyme E180A West Nile virus
0.27
-
 GTP pH 7.5, 37°C, mutant enzyme R170A West Nile virus
0.27
-
 GTP pH 7.5, 37°C, mutant enzyme E180A West Nile virus
0.27
-
 ATP pH 7.5, 37°C, mutant enzyme E182A West Nile virus
0.29
-
 GTP pH 7.5, 37°C, mutant enzyme Q188A West Nile virus
0.33
-
 GTP pH 7.5, 37°C, mutant enzyme D172A West Nile virus
0.34
-
 GTP pH 7.5, 37°C, mutant enzyme E169A West Nile virus
0.35
-
 GTP pH 7.5, 37°C, wild-type enzyme West Nile virus
0.39
-
 ATP pH 7.5, 37°C, mutant enzyme K186A West Nile virus
0.64
-
 ATP pH 7.5, 37°C, mutant enzyme Q188A West Nile virus
0.94
-
 ATP pH 7.5, 37°C, mutant enzyme K187A West Nile virus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O West Nile virus the West Nile virus RNA helicase uses the energy derived from the hydrolysis of nucleotides to separate complementary strands of RNA ADP + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
West Nile virus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 West Nile virus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dideoxy-ATP + H2O 53% of the phosphohydrolase activity with ATP West Nile virus 2',3'-dideoxy-ADP + phosphate
-
 ?
2',3'-dideoxy-GTP + H2O 28% of the phosphohydrolase activity with ATP West Nile virus 2',3'-dideoxy-GDP + phosphate
-
 ?
2'-deoxy-ATP + H2O 62% of the phosphohydrolase activity with ATP West Nile virus 2'-deoxy-ADP + phosphate
-
 ?
2'-deoxy-GTP + H2O 39% of the phosphohydrolase activity with ATP West Nile virus 2'-deoxy-GDP + phosphate
-
 ?
2'-deoxy-L-GTP + H2O 11% of the phosphohydrolase activity with ATP West Nile virus 2'-deoxy-L-GDP + phosphate
-
 ?
2'-fluoro-2'-deoxy-ATP + H2O 63% of the phosphohydrolase activity with ATP West Nile virus 2'-fluoro-2'-deoxy-ADP + phosphate
-
 ?
2'-fluoro-2'-deoxy-GTP + H2O 22% of the phosphohydrolase activity with ATP West Nile virus 2'-fluoro-2'-deoxy-GDP + phosphate
-
 ?
2'-O-methyl-GTP + H2O 24% of the phosphohydrolase activity with ATP West Nile virus 2'-O-methyl-GDP + phosphate
-
 ?
2-amino-ATP + H2O 103% of the phosphohydrolase activity with ATP West Nile virus 2-amino-ADP + phosphate
-
 ?
2-hydroxy-ATP + H2O 40% of the phosphohydrolase activity with ATP West Nile virus 2-hydroxy-ADP + phosphate
-
 ?
3'-deoxy-ATP + H2O 60% of the phosphohydrolase activity with ATP West Nile virus 3'-deoxy-ADP + phosphate
-
 ?
3'-deoxy-GTP + H2O 12% of the phosphohydrolase activity with ATP West Nile virus 3'-deoxy-GDP + phosphate
-
 ?
3'-O-methyl-GTP + H2O 35% of the phosphohydrolase activity with ATP West Nile virus 3'-O-methyl-GDP + phosphate
-
 ?
6-methyl-thio-GTP + H2O 40% of the phosphohydrolase activity with ATP West Nile virus 6-methyl-thio-GDP + phosphate
-
 ?
6-methyl-thio-ITP + H2O 16% of the phosphohydrolase activity with ATP West Nile virus 6-methyl-thio-IDP + phosphate
-
 ?
6-thio-GTP + H2O 93% of the phosphohydrolase activity with ATP West Nile virus 6-thio-GDP + phosphate
-
 ?
7-methyl-GTP + H2O 14% of the phosphohydrolase activity with ATP West Nile virus 7-methyl-GDP + phosphate
-
 ?
8-bromo-ATP + H2O 124% of the phosphohydrolase activity with ATP West Nile virus 8-bromo-ADP + phosphate
-
 ?
8-bromo-GTP + H2O 19% of the phosphohydrolase activity with ATP West Nile virus 8-bromo-GDP + phosphate
-
 ?
8-iodo-GTP + H2O 54% of the phosphohydrolase activity with ATP West Nile virus 8-iodo-GDP + phosphate
-
 ?
ara-ATP + H2O 18% of the phosphohydrolase activity with ATP West Nile virus ara-ADP + phosphate
-
 ?
ATP + H2O the West Nile virus RNA helicase uses the energy derived from the hydrolysis of nucleotides to separate complementary strands of RNA West Nile virus ADP + phosphate
-
 ?
ATP + H2O the amino acids Arg185, Arg202 and Asn417 are critical for phosphohydrolysis West Nile virus ADP + phosphate
-
 ?
GTP + H2O 49% of the phosphohydrolase activity with ATP West Nile virus GDP + phosphate
-
 ?
ITP + H2O 49% of the phosphohydrolase activity with ATP West Nile virus IDP + phosphate
-
 ?
N1-methyl-ATP + H2O 66% of the phosphohydrolase activity with ATP West Nile virus N1-methyl-ADP + phosphate
-
 ?
N1-methyl-GTP + H2O 49% of the phosphohydrolase activity with ATP West Nile virus N1-methyl-GDP + phosphate
-
 ?
N6-methyl-ATP + H2O 43% of the phosphohydrolase activity with ATP West Nile virus N6-methyl-ADP + phosphate
-
 ?
O6-methyl-GTP + H2O 17% of the phosphohydrolase activity with ATP West Nile virus O6-methyl-GDP + phosphate
-
 ?
ribavirin triphosphate + H2O 36% of the phosphohydrolase activity with ATP West Nile virus ribavirin diphosphate + phosphate
-
 ?
XTP + H2O 40% of the phosphohydrolase activity with ATP West Nile virus XDP + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
NS3 protein
-
 West Nile virus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at West Nile virus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.58
-
 ATP pH 7.5, 37°C, mutant enzyme D172A West Nile virus
0.92
-
 GTP pH 7.5, 37°C, mutant enzyme D172A West Nile virus
0.92
-
 ATP pH 7.5, 37°C, mutant enzyme E173A West Nile virus
1
-
 ATP pH 7.5, 37°C, mutant enzyme R170A West Nile virus
1
-
 GTP pH 7.5, 37°C, mutant enzyme K187A West Nile virus
1.1
-
 GTP pH 7.5, 37°C, mutant enzyme E173A West Nile virus
1.1
-
 GTP pH 7.5, 37°C, mutant enzyme F179A West Nile virus
1.1
-
 ATP pH 7.5, 37°C, mutant enzyme E180A West Nile virus
1.2
-
 ATP pH 7.5, 37°C, mutant enzyme F179A West Nile virus
1.25
-
 ATP pH 7.5, 37°C, mutant enzyme E169A West Nile virus
1.3
-
 GTP pH 7.5, 37°C, mutant enzyme E180A West Nile virus
1.7
-
 GTP pH 7.5, 37°C, mutant enzyme R170A West Nile virus
1.7
-
 GTP pH 7.5, 37°C, mutant enzyme K186A West Nile virus
1.7
-
 GTP pH 7.5, 37°C, mutant enzyme Q188A West Nile virus
1.83
-
 GTP pH 7.5, 37°C, mutant enzyme E169A West Nile virus
2.2
-
 ATP pH 7.5, 37°C, mutant enzyme K187A West Nile virus
2.7
-
 ATP pH 7.5, 37°C, mutant enzyme K186A West Nile virus
2.75
-
 GTP pH 7.5, 37°C, wild-type enzyme West Nile virus
3
-
 ATP pH 7.5, 37°C, wild-type enzyme West Nile virus
3.3
-
 ATP pH 7.5, 37°C, mutant enzyme E182A West Nile virus
3.3
-
 ATP pH 7.5, 37°C, mutant enzyme Q188A West Nile virus
3.6
-
 GTP pH 7.5, 37°C, mutant enzyme E182A West Nile virus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at West Nile virus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.2
-
 2-amino-ATP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
0.3
-
 ATP pH 7.5, 37°C, wild-type enzyme West Nile virus
0.3
-
 2',3'-dideoxy-GTP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
0.4
-
 2'-fluoro-2'-deoxy-ATP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
0.5
-
 ara-ATP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
0.5
-
 3'-deoxy-ATP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
0.5
-
 N6-methyl-ATP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
0.6
-
 2',3'-dideoxy-ATP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
0.6
-
 2'-deoxy-ATP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
0.6
-
 6-methyl-thio-ITP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
0.7
-
 8-bromo-ATP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
0.9
-
 ITP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
1
-
 7-methyl-GTP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
1.3
-
 2'-deoxy-L-GTP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
1.5
-
 N1-methyl-ATP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
2.3
-
 ribavirin triphosphate pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
2.4
-
 2-hydroxy-ATP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
2.6
-
 N1-methyl-GTP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
3.4
-
 2'-deoxy-GTP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus
8.1
-
 GTP pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.3
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 2-amino-ATP
0.4
-
 pH 7.5, 37°C, wild-type enzyme West Nile virus ATP
0.5
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 2'-fluoro-2'-deoxy-ATP
0.5
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 2',3'-dideoxy-GTP
0.7
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus ara-ATP
0.7
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 3'-deoxy-ATP
0.7
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus N6-methyl-ATP
0.8
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 2',3'-dideoxy-ATP
0.8
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 6-methyl-thio-ITP
0.9
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 2'-deoxy-ATP
0.9
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 8-bromo-ATP
1.2
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus ITP
1.4
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 7-methyl-GTP
1.8
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 2'-deoxy-L-GTP
2
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus N1-methyl-ATP
2.7
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 6-thio-GTP
3.1
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus ribavirin triphosphate
3.3
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus 2-hydroxy-ATP
3.5
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus N1-methyl-GTP
11
-
 pH 7.5, 37°C, wild-type enzyme, inhibition of the ATPase reaction West Nile virus GTP

General Information

General Information Comment Organism
physiological function the West Nile virus RNA helicase uses the energy derived from the hydrolysis of nucleotides to separate complementary strands of RNA West Nile virus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.7
-
 ATP pH 7.5, 37°C, mutant enzyme E180A West Nile virus
2.3
-
 ATP pH 7.5, 37°C, mutant enzyme K187A West Nile virus
2.79
-
 GTP pH 7.5, 37°C, mutant enzyme D172A West Nile virus
3.22
-
 ATP pH 7.5, 37°C, mutant enzyme D172A West Nile virus
4.6
-
 ATP pH 7.5, 37°C, mutant enzyme E173A West Nile virus
4.8
-
 GTP pH 7.5, 37°C, mutant enzyme E180A West Nile virus
5
-
 ATP pH 7.5, 37°C, mutant enzyme R170A West Nile virus
5.2
-
 ATP pH 7.5, 37°C, mutant enzyme Q188A West Nile virus
5.38
-
 GTP pH 7.5, 37°C, mutant enzyme E169A West Nile virus
5.68
-
 ATP pH 7.5, 37°C, mutant enzyme E169A West Nile virus
5.9
-
 GTP pH 7.5, 37°C, mutant enzyme Q188A West Nile virus
6.3
-
 GTP pH 7.5, 37°C, mutant enzyme R170A West Nile virus
6.7
-
 ATP pH 7.5, 37°C, mutant enzyme F179A West Nile virus
6.9
-
 ATP pH 7.5, 37°C, mutant enzyme K186A West Nile virus
10
-
 GTP pH 7.5, 37°C, mutant enzyme E173A West Nile virus
11
-
 GTP pH 7.5, 37°C, mutant enzyme K187A West Nile virus
12
-
 GTP pH 7.5, 37°C, mutant enzyme F179A West Nile virus
12.2
-
 ATP pH 7.5, 37°C, mutant enzyme E182A West Nile virus
15.7
-
 GTP pH 7.5, 37°C, mutant enzyme E182A West Nile virus
17
-
 GTP pH 7.5, 37°C, mutant enzyme K186A West Nile virus
21.43
-
 ATP pH 7.5, 37°C, wild-type enzyme West Nile virus
22.26
-
 GTP pH 7.5, 37°C, wild-type enzyme West Nile virus