Activating Compound | Comment | Organism | Structure |
---|---|---|---|
DNA | stability of the enzyme's closed clamp conformation may influence DNA-stimulated ATP hydrolysis | Homo sapiens | |
DNA | stability of the enzyme's closed clamp conformation may influence DNA-stimulated ATP hydrolysis | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzyme in yeast JELt1 cells | Homo sapiens |
overexpression of wild-type and mutant enzyme in yeast JELt1 cells | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
Y28F | site-directed mutagenesis, mutation of an ATP hydrolysis domain residue of a bisdioxopiperazine- and sodium orthovanadate-resistant mutant enzyme, mutant shows reduced DNA-dependent ATP-hydrolysis activity without affecting the relaxation activity of the enzyme, no formation of the closed clamp conformation with vanadate or ADP, but with ADP-PNP | Saccharomyces cerevisiae |
Y50F | site-directed mutagenesis, mutation of an ATP hydrolysis domain residue of a bisdioxopiperazine- and sodium orthovanadate-resistant mutant enzyme, mutant shows reduced DNA-dependent ATP-hydrolysis activity without affecting the relaxation activity of the enzyme, no formation of the closed clamp conformation with vanadate or ADP, but with ADP-PNP | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | traps the wild-type enzyme in the closed clamp formation, which shows no ATPase activity | Homo sapiens | |
ADP | traps the wild-type enzyme in the closed clamp formation, which shows no ATPAse hydrolysis activity | Saccharomyces cerevisiae | |
bisdioxopiperazine | inhibits the ATPase activity of the wild-type enzyme | Homo sapiens | |
bisdioxopiperazine | inhibits the ATPase activity of the wild-type enzyme | Saccharomyces cerevisiae | |
sodium orthovanadate | inhibits the ATPase activity of the wild-type enzyme, 50% inhibition at 0.005 mM, traps the enzyme in a salt-stable closed conformation, i.e. the closed clamp, no inhibition of mutant Y50F | Homo sapiens | |
sodium orthovanadate | noncompetitive, formation of a ternary enzyme-ADP-vanadate complex, inhibits the ATPase activity of the wild-type enzyme, traps the enzyme in a salt-stable closed conformation, i.e. the closed clamp, no inhibition of mutant Y28F | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | ATP hydrolysis kinetics for wild-type and Y28F mutant enzyme | Saccharomyces cerevisiae | |
additional information | - |
additional information | ATP hydrolysis kinetics for wild-type and Y50F mutant enzyme | Homo sapiens | |
0.2 | - |
ATP | pH 7.7, 30°C, recombinant Y28F mutant enzyme | Saccharomyces cerevisiae | |
0.3 | - |
ATP | pH 7.7, 30°C, recombinant wild-type enzyme | Saccharomyces cerevisiae | |
0.3 | - |
ATP | pH 7.7, 30°C, recombinant Y50F mutant enzyme | Homo sapiens | |
0.5 | - |
ATP | pH 7.7, 30°C, recombinant wild-type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Homo sapiens | 5634 | - |
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Homo sapiens | |
KCl | - |
Saccharomyces cerevisiae | |
Mg2+ | - |
Homo sapiens | |
Mg2+ | - |
Saccharomyces cerevisiae | |
NaCl | - |
Homo sapiens | |
NaCl | - |
Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme IIalpha | - |
Saccharomyces cerevisiae | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
DNA + ATP | cleavage requires coupling to ATP hydrolysis | Homo sapiens | ? | - |
? | |
DNA + ATP + H2O | relaxation and cleavage of DNA, cleavage requires coupling to ATP hydrolysis | Saccharomyces cerevisiae | DNA + ADP + phosphate | - |
? | |
additional information | the enzyme performs ATP hydrolysis activity | Homo sapiens | ? | - |
? | |
additional information | the enzyme performs ATP hydrolysis activity | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | stability of the enzyme's closed clamp conformation may influence DNA-stimulated ATP hydrolysis | Homo sapiens |
More | stability of the enzyme's closed clamp conformation may influence DNA-stimulated ATP hydrolysis | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
Top2 | - |
Homo sapiens |
Top2 | - |
Saccharomyces cerevisiae |
Top2alpha | - |
Homo sapiens |
Topoisomerase II | - |
Homo sapiens |
Topoisomerase II | - |
Saccharomyces cerevisiae |
topoisomerase IIalpha | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
30 | - |
assay at | Saccharomyces cerevisiae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
ATP | pH 7.7, 30°C, recombinant wild-type or Y50F mutant enzyme, in absence of DNA | Homo sapiens | |
1 | - |
ATP | pH 7.7, 30°C, recombinant Y50F mutant enzyme, in absence of DNA | Saccharomyces cerevisiae | |
1.1 | - |
ATP | pH 7.7, 30°C, recombinant wild-type enzyme, in absence of DNA | Saccharomyces cerevisiae | |
1.2 | - |
ATP | pH 7.7, 30°C, recombinant Y50F mutant enzyme, in presence of DNA | Saccharomyces cerevisiae | |
1.5 | - |
ATP | pH 7.7, 30°C, recombinant Y50F mutant enzyme, in presence of DNA | Homo sapiens | |
3.6 | - |
ATP | pH 7.7, 30°C, recombinant wild-type enzyme, in presence of DNA | Homo sapiens | |
3.6 | - |
ATP | pH 7.7, 30°C, recombinant wild-type enzyme, in presence of DNA | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.7 | - |
assay at, ATP hydrolysis assay | Homo sapiens |
8 | - |
assay at, DNA relaxation assay | Homo sapiens |
8 | - |
assay at, DNA relaxation assay | Saccharomyces cerevisiae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | stability of the enzyme's closed clamp conformation may influence DNA-stimulated ATP hydrolysis | Homo sapiens | |
ATP | stability of the enzyme's closed clamp conformation may influence DNA-stimulated ATP hydrolysis | Saccharomyces cerevisiae |