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Literature summary for 5.6.2.2 extracted from
- DNA methylation impacts the cleavage activity of Chlorella virus topoisomerase II (2005), Biochemistry, 44, 15378-15386.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Paramecium bursaria Chlorella virus 1 |
| expression in Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Chlorella virus Marburg |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | 5-methylcytosine in DNA has a weak effect on enzyme DNA scission activity, 5mC has a 42% content in viral DNA | Chlorella virus Marburg | |
| additional information | 5-methylcytosine in DNA has a weak effect on enzyme DNA scission activity, 5mC has a high content in viral DNA | Paramecium bursaria Chlorella virus 1 | |
| N6-Methyladenine | i.e.6 mA, inhibits DNA scission by the enzyme, 10% content in viral DNA | Chlorella virus Marburg |  |
| N6-Methyladenine | i.e.6mA, inhibits DNA scission by the enzyme, high content in viral DNA | Paramecium bursaria Chlorella virus 1 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | DNA cleavage kinetics | Paramecium bursaria Chlorella virus 1 | |
| additional information | - |
additional information | DNA cleavage kinetics | Chlorella virus Marburg |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | - |
Paramecium bursaria Chlorella virus 1 | |
| KCl | - |
Chlorella virus Marburg | |
| Mg2+ | required for cleavage | Paramecium bursaria Chlorella virus 1 | |
| Mg2+ | required for cleavage | Chlorella virus Marburg | |
| NaCl | - |
Paramecium bursaria Chlorella virus 1 | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dsDNA + ATP + H2O | Paramecium bursaria Chlorella virus 1 | breakage, passage, decatenation, and rejoining of dsDNA | ? | - |
? | |
| dsDNA + ATP + H2O | Chlorella virus Marburg | breakage, passage, decatenation, and rejoining of dsDNA | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chlorella virus Marburg | - |
i.e. CVM-1 | - |
| Paramecium bursaria Chlorella virus 1 | - |
i.e. PBCV-1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant viral enzyme from Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Paramecium bursaria Chlorella virus 1 |
| recombinant viral enzyme from Saccharomyces cerevisiae strain JEL-1DELTAtop1 | Chlorella virus Marburg |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CVM-1 viral DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of CVM-1 viral DNA, viral DNA contains 10% N6-methyladenine and 42% 5-methylcytosine, 4fold decreased activity compared to unmodified DNA | Chlorella virus Marburg | ? | - |
? | |
| dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
| dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA | Chlorella virus Marburg | ? | - |
? | |
| dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
| dsDNA + ATP + H2O | breakage, passage, decatenation, and rejoining of dsDNA, very high DNA cleavage activity of the viral type enzyme | Chlorella virus Marburg | ? | - |
? | |
| additional information | the enzyme binds unmodified DNA and DNA containing N6-methyladenine and 5-methylcytosine to the same extent | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
| additional information | the enzyme binds unmodified DNA and DNA containing N6-methyladenine and 5-methylcytosine to the same extent | Chlorella virus Marburg | ? | - |
? | |
| PBCV-1 viral DNA + ATP + H2O | breakage, passage, decatenation, and rejoining of PBCV-1 viral DNA, viral DNA contains N6-methyladenine and 5-methylcytosine, decreased activity compared to unmodified DNA | Paramecium bursaria Chlorella virus 1 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Topoisomerase II | - |
Paramecium bursaria Chlorella virus 1 |
| Topoisomerase II | - |
Chlorella virus Marburg |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Paramecium bursaria Chlorella virus 1 |
| 30 | - |
assay at | Chlorella virus Marburg |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Paramecium bursaria Chlorella virus 1 |
| 8.5 | - |
assay at | Chlorella virus Marburg |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Paramecium bursaria Chlorella virus 1 | |
| ATP | - |
Chlorella virus Marburg | |
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