Activating Compound | Comment | Organism | Structure |
---|---|---|---|
vanadate | slight activation of mutant L169F | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
overexpression of wild-type and mutant enzymes in Saccharomyces cerevisiae strain JelDELTATop1 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
L169F | site-directed mutagenesis, the mutant enzyme is highly resistant to vanadate, the mutant shows reduced DNA passage activity compared to the wild-type enzyme | Homo sapiens |
L169I | site-directed mutagenesis, the mutant shows unaltered DNA passage activity and slightly reduced DNA hyperstimulation compared to the wild-type enzyme | Homo sapiens |
R162K | site-directed mutagenesis, the mutant enzyme is highly resistant to vanadate, but shows only slightly reduced sensitivity to bisdioxopiperazine compared to wild-type enzyme, reduced DNA hyperstimulation and DNA passage activity compared to the wild-type enzyme compared to the wild-type enzyme | Homo sapiens |
R162Q | site-directed mutagenesis, the mutant enzyme is highly resistant to vanadate, the mutant shows no DNA hyperstimulation, nearly no ATPase activity, and reduced DNA passage activity and DNA hyperstimulation compared to the wild-type enzyme | Homo sapiens |
Y165S | site-directed mutagenesis, the mutant enzyme is highly resistant to vanadate, the mutant shows no DNA hyperstimulation, nearly no ATPase activity, and highly reduced DNA passage activity and DNA hyperstimulation compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bisdioxopiperazine | inhibition of wild-type enzyme and mutant R162K, inhibition mechanism | Homo sapiens | |
vanadate | inhibition of wild-type enzyme, no inhibition of mutant R162K, R162Q and Y165S, inhibition mechanism | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.21 | - |
DNA | pH 7.7, 37°C, DNA passage, mutant R162Q | Homo sapiens | |
0.3 | - |
DNA | pH 7.7, 37°C, DNA passage, mutant Y165S | Homo sapiens | |
0.39 | - |
DNA | pH 7.7, 37°C, DNA passage, wild-type enzyme | Homo sapiens | |
0.55 | - |
DNA | pH 7.7, 37°C, DNA passage, mutant L169I | Homo sapiens | |
0.61 | - |
DNA | pH 7.7, 37°C, DNA passage, mutant R162K | Homo sapiens | |
0.94 | - |
DNA | pH 7.7, 37°C, DNA passage, mutant L169F | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | required for DNA passage activity | Homo sapiens | |
Mg2+ | required for ATPase and DNA passage activities | Homo sapiens | |
NaCl | required for DNA passage activity | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isozyme alpha | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
DNA hyperstimulation rates and ATPase activities of wild-type and mutant enzymes, overview | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
dsDNA + ATP + H2O | passage of dsDNA | Homo sapiens | ? | - |
? | |
additional information | the enzyme has intrinsic ATPase activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Topoisomerase II | - |
Homo sapiens |
topoisomerase IIalpha | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
DNA | pH 7.7, 37°C, DNA passage, mutant Y165S | Homo sapiens | |
1.24 | - |
DNA | pH 7.7, 37°C, DNA passage, mutant R162Q | Homo sapiens | |
1.65 | - |
DNA | pH 7.7, 37°C, DNA passage, mutant L169F | Homo sapiens | |
1.8 | - |
DNA | pH 7.7, 37°C, DNA passage, mutant R162K | Homo sapiens | |
3.33 | - |
DNA | pH 7.7, 37°C, DNA passage, mutant L169I | Homo sapiens | |
3.38 | - |
DNA | pH 7.7, 37°C, DNA passage, wild-type enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
ATPase assay at | Homo sapiens |
7.7 | - |
DNA passage assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |