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Literature summary for 5.6.2.2 extracted from

  • Low, R.L.; Orton, S.; Friedman, D.B.
    A truncated form of DNA topoisomerase IIbeta associates with the mtDNA genome in mammalian mitochondria (2003), Eur. J. Biochem., 270, 4173-4186.
    View publication on PubMed
Search for more literature for 5.6.2.2

Inhibitors

Inhibitors Comment Organism Structure
4'-(9-acridinylamine)methanesulfon-m-anisidide 0.003 mM, 50% inhibition Bos taurus
Ethidium bromide 0.6 mg/l, 50% inhibition Bos taurus
etoposide 0.07 mM, 50% inhibition Bos taurus
NEM 0.06 mM, 50% inhibition Bos taurus
novobiocin 0.07 mM, 50% inhibition Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion the mitochondrial enzyme is a truncated version of DNA topoisomerase IIbeta Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ maximal activity at 7.5 mM, required for relaxation of supercoiled DNA Bos taurus
NaCl maximal activity at 0.1 mM Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
 glycerol gradient centrifugation Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
negatively supercoiled DNA + ATP + H2O relaxation Bos taurus ?
-
 ?
topoligically relaxed plasmid DNA + ATP catenation in presence of a DNA crowding agent Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 150000, SDS-PAGE Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Bos taurus