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Literature summary for 5.6.2.2 extracted from
- DNA unknotting activity (DNA topoisomerase II) isolated from a thermophilic archaebacterium Sulfolobus is inhibited by novobiocin. Partial purification, identification of the two subunits and characteristics of the enzyme (1994), Biochim. Biophys. Acta, 1219, 107-114.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine | - |
Sulfolobus sp. |  |
| ADP | - |
Sulfolobus sp. | |
| coumermycin | coumermycin A | Sulfolobus sp. | |
| CTP | - |
Sulfolobus sp. | |
| dATP | - |
Sulfolobus sp. | |
| GTP | - |
Sulfolobus sp. | |
| NaCl | above 300 mM | Sulfolobus sp. | |
| novobiocin | - |
Sulfolobus sp. | |
| phosphate | - |
Sulfolobus sp. | |
| UTP | - |
Sulfolobus sp. | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | ATP-dependent DNA relaxing activity requires the presence of at least 1 mM MgCl2 | Sulfolobus sp. | |
| NaCl | required at concentrations of 50 mM | Sulfolobus sp. | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 65000 | - |
x * 65000, B-subunit with the ATP-binding site, + x * 85000, A-subunit with nicking-closing activity | Sulfolobus sp. |
| 85000 | - |
x * 65000, B-subunit with the ATP-binding site, + x * 85000, A-subunit with nicking-closing activity | Sulfolobus sp. |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Sulfolobus sp. | - |
- |
- |
| Sulfolobus sp. B12 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Sulfolobus sp. |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| network of DNA rings + ATP + H2O | decatenation | Sulfolobus sp. | monomeric DNA circles + ADP + phosphate | - |
? | |
| network of DNA rings + ATP + H2O | decatenation | Sulfolobus sp. B12 | monomeric DNA circles + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | catenation | Sulfolobus sp. | catenated DNA networks + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | catenation | Sulfolobus sp. B12 | catenated DNA networks + ADP + phosphate | - |
? | |
| supercoiled DNA + ATP + H2O | relaxation | Sulfolobus sp. | relaxed DNA + ADP + phosphate | ADP is cleaved to ADP and phosphate | ? | |
| supercoiled DNA + ATP + H2O | ATP-dependent relaxation of negative and positive supercoils | Sulfolobus sp. | relaxed DNA + ADP + phosphate | ADP is cleaved to ADP and phosphate | ? | |
| supercoiled DNA + ATP + H2O | relaxation | Sulfolobus sp. B12 | relaxed DNA + ADP + phosphate | ADP is cleaved to ADP and phosphate | ? | |
| supercoiled DNA + ATP + H2O | ATP-dependent relaxation of negative and positive supercoils | Sulfolobus sp. B12 | relaxed DNA + ADP + phosphate | ADP is cleaved to ADP and phosphate | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 65000, B-subunit with the ATP-binding site, + x * 85000, A-subunit with nicking-closing activity | Sulfolobus sp. |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Sulfolobus sp. |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | 85 | no DNA relaxing activity below 75°C and above 80°C | Sulfolobus sp. |
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