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Literature summary for 5.6.2.2 extracted from

  • Bergerat, A.; Gadelle, D.; Forterre, P.
    Purification of a DNA topoisomerase II from the hyperthermophilic archaeon Sulfolobus shibatae (1994), J. Biol. Chem., 269, 27663-27669.
    View publication on PubMed
Search for more literature for 5.6.2.2

Activating Compound

Activating Compound Comment Organism Structure
spermidine stimulates Saccharolobus shibatae

Inhibitors

Inhibitors Comment Organism Structure
(1S,3S)-3-acetyl-3,5,12-trihydroxy-10-methoxy-6,11-dioxo-1,2,3,4,6,11-hexahydrotetracen-1-yl 3-amino-2,3,6-trideoxy-alpha-L-lyxo-hexopyranoside
-
 Saccharolobus shibatae
4'-(9-acridinylamino)methansulfon-m-anisidide
-
 Saccharolobus shibatae
4'-(9-acridinylamino)methansulfon-o-anisidide
-
 Saccharolobus shibatae
Amsarcines
-
 Saccharolobus shibatae
coumermycin no inhibition Saccharolobus shibatae
doxorubicin
-
 Saccharolobus shibatae
ellipticines
-
 Saccharolobus shibatae
NaCl above 100 mM Saccharolobus shibatae
novobiocin no inhibition Saccharolobus shibatae
quinolones e.g. CP-115,953 Saccharolobus shibatae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharolobus shibatae
Mg2+ Mg2+ is effective in the concentration range 1-40 mM, optimal concentration is around 30 mM Saccharolobus shibatae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 2 * 47000 + 2 * 60000, SDS-PAGE Saccharolobus shibatae
60000
-
 2 * 47000 + 2 * 60000, SDS-PAGE Saccharolobus shibatae
230000
-
 gel filtration, sucrose density gradient centrifugation Saccharolobus shibatae

Organism

Organism UniProt Comment Textmining
Saccharolobus shibatae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Saccharolobus shibatae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Saccharolobus shibatae

Storage Stability

Storage Stability Organism
4°C, 50 mM potassium phosphate buffer, 1 mM EDTA, 1 mM DTT, 0.0005% Triton X-100 Saccharolobus shibatae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information changes the linking number of a closed circular molecule by an increment of two Saccharolobus shibatae ?
-
 ?
network of DNA rings + ATP + H2O decatenation Saccharolobus shibatae monomeric DNA circles + ADP + phosphate
-
 ?
network of DNA rings + ATP + H2O enzyme is more efficient in decatenation than relaxation Saccharolobus shibatae monomeric DNA circles + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O catenation Saccharolobus shibatae catenated DNA networks + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O relaxation Saccharolobus shibatae relaxed DNA + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O ATP-dependent relaxation of negative and positive supercoils Saccharolobus shibatae relaxed DNA + ADP + phosphate
-
 ?

Subunits

Subunits Comment Organism
tetramer 2 * 47000 + 2 * 60000, SDS-PAGE Saccharolobus shibatae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70 80
-
 Saccharolobus shibatae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 80 50°C: no activity, 70-80°C: maximal activity Saccharolobus shibatae

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
 pH 7, enzyme concentration 90 ng/ml, less than 15% loss of activity after 40 min Saccharolobus shibatae
80
-
 pH 7, enzyme concentration 90 ng/ml, half-life: about 35 min. At an enzyme concentration of 900 ng/ml less than 20% loss of activity after 40 min Saccharolobus shibatae
95
-
 pH 7, enzyme concentration 90 ng/ml, more than 90% inactivation after 5 min Saccharolobus shibatae