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Literature summary for 5.6.2.2 extracted from

  • Benedetti, P.; Baldi, M.I.; Mattoccia, E.; Tocchini-Valentini, G.P.
    Purification and characterization of Xenopus laevis topoisomerase II (1983), EMBO J., 2(8), 1303-1308.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 5.6.2.2

Activating Compound

Activating Compound Comment Organism Structure
spermidine stimulates catenation reaction Xenopus laevis

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus
-
 Xenopus laevis 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
K+ catenation reaction is very sensitive to ionic strength, being optimal in the range 20-30 mM KCl or 20-50 mM NH4Cl Xenopus laevis
Mg2+ required Xenopus laevis
NH4Cl catenation reaction is very sensitive to ionic strength, being optimal in the range 20-30 mM KCl or 20-50 mM NH4Cl Xenopus laevis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
175000
-
 sucrose density gradient centrifugation Xenopus laevis

Organism

Organism UniProt Comment Textmining
Xenopus laevis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Xenopus laevis

Source Tissue

Source Tissue Comment Organism Textmining
oocyte
-
 Xenopus laevis
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Xenopus laevis

Storage Stability

Storage Stability Organism
-70°C, partially purified enzyme is stable for at least 3 months. Purified enzyme loses 50% of its activity after 3 d Xenopus laevis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information ATP hydrolysis Xenopus laevis ?
-
 ?
network of DNA rings + ATP + H2O decatenation Xenopus laevis monomeric DNA circles + ADP + phosphate
-
 ?
supercoiled DNA + ATP + H2O catenation Xenopus laevis catenated DNA networks + ADP + phosphate in presence of spermine circular DNA is catenated to dimers, trimer and a network ?
supercoiled DNA + ATP + H2O relaxation Xenopus laevis relaxed DNA + ADP + phosphate
-
 ?