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Literature summary for 5.6.2.2 extracted from

  • Goto, T.; Laipis, P.; Wang, J.C.
    The purification and characterization of DNA topoisomerases I and II of the yeast Saccharomyces cerevisiae (1984), J. Biol. Chem., 259, 10422-10429.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Adenylyl imidodiphosphate
-
Saccharomyces cerevisiae
adenylyl(beta,gamma-methylene)diphosphate
-
Saccharomyces cerevisiae
Berenil
-
Saccharomyces cerevisiae
coumermycin
-
Saccharomyces cerevisiae
Ethidium bromide
-
Saccharomyces cerevisiae
NEM 0.5 mM Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion no topoisomerase specific for mitochondria Saccharomyces cerevisiae 5739
-
nucleus
-
Saccharomyces cerevisiae 5634
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ absolute requirement for a divalent cation, Mg2+ is the most effective. Mn2+, Ca2+, or Co2+ support the reaction to a lesser extent Saccharomyces cerevisiae
Co2+ absolute requirement for a divalent cation, Mg2+ is the most effective. Mn2+, Ca2+, or Co2+ support the reaction to a lesser extent Saccharomyces cerevisiae
Mg2+ absolute requirement for a divalent cation, Mg2+ is the most effective. Mn2+, Ca2+, or Co2+ support the reaction to a lesser extent Saccharomyces cerevisiae
Mn2+ absolute requirement for a divalent cation, Mg2+ is the most effective. Mn2+, Ca2+, or Co2+ support the reaction to a lesser extent Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
2 * 150000, SDS-PAGE Saccharomyces cerevisiae
265000
-
gel filtration, glycerol density gradient centrifugation Saccharomyces cerevisiae

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information dATP can substitute for ATP Saccharomyces cerevisiae ?
-
?
additional information changes the linking number of a closed circular molecule by integers Saccharomyces cerevisiae ?
-
?
network of DNA rings + ATP + H2O decatenation Saccharomyces cerevisiae monomeric DNA circles + ADP + phosphate
-
?
supercoiled DNA + ATP + H2O catenation Saccharomyces cerevisiae catenated DNA networks + ADP + phosphate
-
?
supercoiled DNA + ATP + H2O relaxation Saccharomyces cerevisiae relaxed DNA + ADP + phosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 150000, SDS-PAGE Saccharomyces cerevisiae