Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Adenylyl imidodiphosphate | - |
Saccharomyces cerevisiae | |
adenylyl(beta,gamma-methylene)diphosphate | - |
Saccharomyces cerevisiae | |
Berenil | - |
Saccharomyces cerevisiae | |
coumermycin | - |
Saccharomyces cerevisiae | |
Ethidium bromide | - |
Saccharomyces cerevisiae | |
NEM | 0.5 mM | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | no topoisomerase specific for mitochondria | Saccharomyces cerevisiae | 5739 | - |
nucleus | - |
Saccharomyces cerevisiae | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | absolute requirement for a divalent cation, Mg2+ is the most effective. Mn2+, Ca2+, or Co2+ support the reaction to a lesser extent | Saccharomyces cerevisiae | |
Co2+ | absolute requirement for a divalent cation, Mg2+ is the most effective. Mn2+, Ca2+, or Co2+ support the reaction to a lesser extent | Saccharomyces cerevisiae | |
Mg2+ | absolute requirement for a divalent cation, Mg2+ is the most effective. Mn2+, Ca2+, or Co2+ support the reaction to a lesser extent | Saccharomyces cerevisiae | |
Mn2+ | absolute requirement for a divalent cation, Mg2+ is the most effective. Mn2+, Ca2+, or Co2+ support the reaction to a lesser extent | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
2 * 150000, SDS-PAGE | Saccharomyces cerevisiae |
265000 | - |
gel filtration, glycerol density gradient centrifugation | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | dATP can substitute for ATP | Saccharomyces cerevisiae | ? | - |
? | |
additional information | changes the linking number of a closed circular molecule by integers | Saccharomyces cerevisiae | ? | - |
? | |
network of DNA rings + ATP + H2O | decatenation | Saccharomyces cerevisiae | monomeric DNA circles + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | catenation | Saccharomyces cerevisiae | catenated DNA networks + ADP + phosphate | - |
? | |
supercoiled DNA + ATP + H2O | relaxation | Saccharomyces cerevisiae | relaxed DNA + ADP + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 150000, SDS-PAGE | Saccharomyces cerevisiae |