Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ATP | physiological concentration | Bos taurus | |
NEM | - |
Bos taurus | |
novobiocin | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | membrane-associated | Bos taurus | 5739 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates equally effective as Mg2+ | Bos taurus | |
K+ | stimulates, optimal activity at 120 mM | Bos taurus | |
Mg2+ | no absolute requirement for divalent cation. 10 mM Mg2+ stimulates 5fold to 10fold | Bos taurus | |
NH4+ | stimulates | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
gel filtration, glycerol density gradient centrifugation, monomeric form exists at low ionic strength | Bos taurus |
145000 | - |
gel filtration, glycerol density gradient centrifugation, weakly associated dimer exists at high ionic strength | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
calf | - |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
thymus | - |
Bos taurus | - |
Storage Stability | Organism |
---|---|
-20°C, 0.3 M KCl, 40 mM Tris-HCl, pH 7.5, 50% glycerol, stable for almost 1 year | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
supercoiled DNA | relaxation | Bos taurus | relaxed closed circular DNA | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
10 min, in absence of DNA, quickly inactivated | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | pH 5: 25% of maximal activity, pH 6: about 50% of maximal activity, pH 8: about 50% of maximal activity | Bos taurus |