Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.2.1 extracted from

  • Lazarus, G.M.; Henrich, J.P.; Kelly, W.G.; Schmitz, S.A.; Castora, F.J.
    Purification and characterization of a type I DNA topoisomerase from calf thymus mitochondria (1987), Biochemistry, 26, 6195-6203.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ATP physiological concentration Bos taurus
NEM
-
Bos taurus
novobiocin
-
Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion membrane-associated Bos taurus 5739
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ stimulates equally effective as Mg2+ Bos taurus
K+ stimulates, optimal activity at 120 mM Bos taurus
Mg2+ no absolute requirement for divalent cation. 10 mM Mg2+ stimulates 5fold to 10fold Bos taurus
NH4+ stimulates Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
64000
-
gel filtration, glycerol density gradient centrifugation, monomeric form exists at low ionic strength Bos taurus
145000
-
gel filtration, glycerol density gradient centrifugation, weakly associated dimer exists at high ionic strength Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
calf
-

Purification (Commentary)

Purification (Comment) Organism
-
Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
Bos taurus
-

Storage Stability

Storage Stability Organism
-20°C, 0.3 M KCl, 40 mM Tris-HCl, pH 7.5, 50% glycerol, stable for almost 1 year Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
supercoiled DNA relaxation Bos taurus relaxed closed circular DNA
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Bos taurus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
10 min, in absence of DNA, quickly inactivated Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bos taurus

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5: 25% of maximal activity, pH 6: about 50% of maximal activity, pH 8: about 50% of maximal activity Bos taurus