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Literature summary for 5.6.1.8 extracted from
- Myosin surface loop 4 modulates inhibition of actomyosin 1b ATPase activity by tropomyosin (2007), Biochemistry, 46, 2779-2786.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| actin | - |
Mammalia |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| for expression in insect amd mammalian cells | Mammalia |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| Myo1b-GAGEGA | Myo1b in which wild-type loop 4 is replaced with loop 4 of Dictyostelium myosin II, GAGEGA | Mammalia |
| Myo1b-NGLD | Myo1b in which wild-type loop 4 is truncated to NGLD | Mammalia |
| Myo1b1IQ-GAGEGA | Myo1b1IQ in which wild-type loop 4 is replaced with loop 4 of Dictyostelium myosin II, GAGEGA | Mammalia |
| Myo1b1IQ-NGLD | Myo1b1IQ in which wild-type loop 4 is truncated to NGLD | Mammalia |
| Myo1b1IQ-WT | wild-type Myo1b truncated after the first IQ domain at amino acid 728 containing the wild-type loop 4 sequence, RMNGLDES | Mammalia |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| tropomyosin | - |
Mammalia | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
ATP | Myo1b1IQ-GAGEGA, pCa 8.9 | Mammalia | |
| 0.009 | - |
ATP | Myo1b1IQ-GAGEGA, pCa 4.6 with tropomyosin-2 | Mammalia | |
| 0.009 | - |
ATP | Myo1b1IQ-NGLD, pCa 4.6 | Mammalia | |
| 0.009 | - |
ATP | Myo1b1IQ-WT, pCa 4.6 | Mammalia | |
| 0.009 | - |
ATP | Myo1b1IQ-WT, pCa 8.9 | Mammalia | |
| 0.01 | - |
ATP | Myo1b1IQ-GAGEGA, pCa 4.6 | Mammalia | |
| 0.011 | - |
ATP | Myo1b1IQ-NGLD, pCa 8.9 | Mammalia | |
| 0.011 | - |
ATP | Myo1b1IQ-WT, pCa 4.6 with tropomyosin-2 | Mammalia | |
| 0.013 | - |
ATP | Myo1b1IQ-NGLD, pCa 4.6 with tropomyosin-2 | Mammalia | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mammalia | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + myosin bound to actin filament at position n | Mammalia | - |
ADP + phosphate + myosin bound to actin filament at position n+1 | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mammalia | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + myosin bound to actin filament at position n | - |
Mammalia | ADP + phosphate + myosin bound to actin filament at position n+1 | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| actin activated myosin ATPase | - |
Mammalia |
| actomyosin 1b ATPase | - |
Mammalia |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
activity assay | Mammalia |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
activity assay | Mammalia |
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