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Literature summary for 5.6.1.7 extracted from
- Functional characterization of the recombinant group II chaperonin alpha from Thermoplasma acidophilum (2008), J. Biochem., 143, 505-515.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| NH4+ | - |
Thermoplasma acidophilum |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli BL21 | Thermoplasma acidophilum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | divalent cation required for ATP hydrolysis, mediate nucleoside triphosphate-dependent refolding of malate dehydrogenase | Thermoplasma acidophilum | |
| Mg2+ | highest ATP hydrolysis activity, mediate ATP-dependent refolding of malate dehydrogenase and green fluorescent protein, suppression of rhodanese and luciferase thermal aggregation | Thermoplasma acidophilum | |
| Mn2+ | divalent cation required for ATP hydrolysis, mediate ATP-dependent refolding of malate dehydrogenase and green fluorescent protein, suppression of rhodanese and luciferase thermal aggregation, increased substrate protein binding of Ta-cpn alpha | Thermoplasma acidophilum | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoplasma acidophilum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoplasma acidophilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | 60°C, assay at pH 7.0, 90 min, 0,12 mM released phosphate | Thermoplasma acidophilum | ADP + phosphate + an unfolded polypeptide | - |
? | |
| ATP + H2O + a folded polypeptide | folding of green fluorescent protein, 50°C, assay at pH 7.0 | Thermoplasma acidophilum | ADP + phosphate + an unfolded polypeptide | - |
? | |
| ATP + H2O + a folded polypeptide | folding of malate dehydrogenase, 50°C, assay at pH 7.0 | Thermoplasma acidophilum | ADP + phosphate + an unfolded polypeptide | - |
? | |
| CTP + H2O + a folded polypeptide | 5 mM Mg2+, 90 min, 0.1 mM released phosphate | Thermoplasma acidophilum | CDP + phosphate + an unfolded polypeptide | - |
? | |
| GTP + H2O + a folded polypeptide | 5 mM Mg2+, 0.07 mM released phosphate after 90 min | Thermoplasma acidophilum | GDP + phosphate + an unfolded polypeptide | - |
? | |
| additional information | additional ATP-dependent protein refolding activity and suppression of rhodanese and luciferase thermal aggregation | Thermoplasma acidophilum | ? | - |
? | |
| UTP + H2O + a folded polypeptide | 5 mM Mg2+, 90 min, 0.11 mM released phosphate | Thermoplasma acidophilum | UDP + phosphate + an unfolded polypeptide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| archael group II chaperonin | - |
Thermoplasma acidophilum |
| chaperonin alpha subunit | - |
Thermoplasma acidophilum |
| Ta-cpn alpha | - |
Thermoplasma acidophilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
refolding of malate dehydrogenase or green fluorescent protein, suppression of rhodanese and luciferase thermal aggregation | Thermoplasma acidophilum |
| 60 | - |
nucleotide hydrolysis activity | Thermoplasma acidophilum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 70 | 0.15 mM released phosphate at 40°C, 0.35 mM released phosphate at 70°C | Thermoplasma acidophilum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Thermoplasma acidophilum |
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Release 2023.1 (January 2023)
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