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Literature summary for 5.6.1.7 extracted from
- Properties of the chaperonin complex from the halophilic archaeon Haloferax volcanii (2002), FEBS Lett., 532, 309-312.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NaCl | approx. 50% inhibition of CTT complex at 200 mM, approx. 80% inhibition at 800 mM | Haloferax volcanii |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | strong activation of CTT complex, almost no activity at 800 mM, maximal activity above 3.2 M | Haloferax volcanii | |
| Mg2+ | absolutely required for activity of CTT complex | Haloferax volcanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 76200 | - |
x * 76200, immunoblot | Haloferax volcanii |
| 86300 | - |
x * 86300, immunoblot | Haloferax volcanii |
| 95400 | - |
x * 95400, immunoblot | Haloferax volcanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | Haloferax volcanii | - |
ADP + phosphate + an unfolded polypeptide | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Haloferax volcanii | O30560 | - |
- |
| Haloferax volcanii | O30561 | - |
- |
| Haloferax volcanii | Q9HHA2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Haloferax volcanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O + a folded polypeptide | - |
Haloferax volcanii | ADP + phosphate + an unfolded polypeptide | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 76200, immunoblot | Haloferax volcanii |
| ? | x * 86300, immunoblot | Haloferax volcanii |
| ? | x * 95400, immunoblot | Haloferax volcanii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CCT1 | thermosome subunit 1 | Haloferax volcanii |
| CCT2 | thermosome subunit 2, has weak ATPase activity | Haloferax volcanii |
| CCT3 | - |
Haloferax volcanii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Haloferax volcanii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 85 | CTT complex, approx. 50% of maximal activity at 70°C, approx. 80% of maximal activity at 75°C, in the presence of 2.9 N KCl | Haloferax volcanii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
no loss of activity after 30 min | Haloferax volcanii |
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