Literature summary for 5.6.1.5 extracted from

Diao, W.; Yang, X.; Zhou, H.
Purification, crystallization and preliminary X-ray data collection of the N-terminal domain of the 26S proteasome regulatory subunit p27 and its complex with the ATPase domain of Rpt5 from Mus musculus (2014), Acta crystallogr. Sect. F, 70, 611-615.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant expression of His-tagged N-terminus of p27 (residues 1-128) and the C-terminal ATPase domain of Rpt5 (residues 173-442) in a mixed cultre of tranformed Escherichia coli strain BL21(DE3) cells	Mus musculus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant N-terminus of p27 (residues 1-128) in complex with the C-terminal ATPase domain of Rpt5 (residues 173-442), sitting drop method, mixing of 0.001 ml of 4 mg/ml protein in 10 mM HEPES pH 7.5, 50 mM NaCl, 1 mM EDTA, 1 mM DTT, with 0.001 ml of precipitant solution containing 2.0 M NaCl, 10% PEG 6000, X-ray diffraction structure determination and analysis at 1.7 A and 4.0 A resolution, respectively	Mus musculus

Crystallization (Comment)

Organism

purified recombinant N-terminus of p27 (residues 1-128) in complex with the C-terminal ATPase domain of Rpt5 (residues 173-442), sitting drop method, mixing of 0.001 ml of 4 mg/ml protein in 10 mM HEPES pH 7.5, 50 mM NaCl, 1 mM EDTA, 1 mM DTT, with 0.001 ml of precipitant solution containing 2.0 M NaCl, 10% PEG 6000, X-ray diffraction structure determination and analysis at 1.7 A and 4.0 A resolution, respectively

Mus musculus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + H2O + polypeptide	Mus musculus	-	ADP + phosphate + unfolded polypeptide	-	?

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant expression of His-tagged C-terminal ATPase domain of Rpt5 (residues 173-442) from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, and gel filtration as protein complex with the N-terminus of p27	Mus musculus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O + polypeptide	-	Mus musculus	ADP + phosphate + unfolded polypeptide	-	?
ATP + H2O + polypeptide	C-terminal ATPase domain of Rpt5, residues 173-442	Mus musculus	ADP + phosphate + unfolded polypeptide	-	?

Synonyms

Synonyms	Comment	Organism
26S protease regulatory subunit 6A	-	Mus musculus
Rpt5	-	Mus musculus

General Information

General Information	Comment	Organism
metabolism	the RP is composed of two subcomplexes: the base and lid. Three non-ATPase subunits, Rpn1, Rpn2 and Rpn13, and six AAA-ATPase subunits, Rpt1-6, which contain four helices at their C-termini, form the base that is attached to the CP alpha-ring, and other non-ATPase subunits form the lid. Rpt5, i.e. 26S protease regulatory subunit 6A, is one of the six ATPase subunits of the regulatory particle, RP, one of two subcomplexes of the 26S proteasome	Mus musculus