Literature summary for 5.6.1.5 extracted from

Kirkland, P.A.; Gil, M.A.; Karadzic, I.M.; Maupin-Furlow, J.A.
Genetic and proteomic analyses of a proteasome-activating nucleotidase A mutant of the haloarchaeon Haloferax volcanii (2008), J. Bacteriol., 190, 193-205.

Search for more literature for 5.6.1.5

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli, strains DH5alpha and GM2163, pCR-BluntII-TOPO to generate plasmid pJAM636, transformed into Haloferax volcanii DS70, recombinant strain, plasmids pJAM650, pJAM1012	Haloferax volcanii

Organism

Organism	UniProt	Comment	Textmining
Haloferax volcanii	D4GUJ7	panA gene product; isogenic mutant strain of panA	-

Purification (Commentary)

Purification (Comment)	Organism
gel filtration	Haloferax volcanii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	comparative proteomic analysis of wild-type cells and those lacking proteasomal function through deletion of the proteasome-activating nucleotidase PanA, reduced growth rate and cell yield of mutant strain, enriched proteins exclusive to or more abundant in the panA mutant includes cell division, dihydroxyacetone kinase-linked phosphoenolpyruvate phosphotransferase and oxidoreductase homologs, differences in transcriptional regulation and signal transduction proteins, proteasome deficiency causes an up-regulation of stress responses, components of the Fe-S cluster assembly, protein-folding, DNA binding and repair, oxidative and osmotic stress, phosphorus assimilation, and polyphosphate synthesis systems enriched in panA mutants	Haloferax volcanii

Synonyms

Synonyms	Comment	Organism
PanA	-	Haloferax volcanii
proteasome-activating nucleotidase A	-	Haloferax volcanii

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Release 2023.1 (January 2023)