Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 5.6.1.4 extracted from

  • Shimizu, T.; Toyoshima, Y.Y.; Edamatsu, M.; Vale, R.D.
    Comparison of the motile and enzymatic properties of two microtubule minus-end-directed motors, ncd and cytoplasmic dynein (1995), Biochemistry, 34, 1575-1582.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
microtubule in presence of microtubule, ncd ATPase activity is stimulated by ca. 30fold Drosophila sp. (in: flies)
microtubules stimulates ncd ATPase activity by ca. 30fold Drosophila melanogaster

Cloned(Commentary)

Cloned (Comment) Organism
PCR from a ncd cDNA, ncd cloned, polypeptide expressed in Escherichia coli Drosophila melanogaster

Inhibitors

Inhibitors Comment Organism Structure
additional information in contrast to cytoplasmic dynein, ncd polypeptide is not cleaved by UV-vanadate treatment, and its ATPase and motility are unaffected by vanadate (up to 0.1 mM) Drosophila sp. (in: flies)
V5+ dynein Drosophila melanogaster

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Bos taurus 5737
-
microtubule
-
Drosophila sp. (in: flies) 5874
-

Metals/Ions

Metals/Ions Comment Organism Structure
NaCl maximal stimulation at 0.15-0.2 M Drosophila sp. (in: flies)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
73000
-
ncd polypeptide, SDS-PAGE Drosophila melanogaster
80000
-
ncd Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + a kinesin associated with a microtubule at position n Drosophila melanogaster
-
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
ATP + H2O + a kinesin associated with a microtubule at position n Bos taurus
-
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
ATP + H2O + a kinesin associated with a microtubule at position n Doryteuthis pealeii
-
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
ATP + H2O + a kinesin associated with a microtubule at position n Tetrahymena sp.
-
ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
bovine
-
Doryteuthis pealeii
-
squid
-
Drosophila melanogaster
-
-
-
Drosophila sp. (in: flies)
-
recombinantly expressed in Escherichia coli
-
Tetrahymena sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial, recombinant protein, expressed in Escherichia coli Drosophila melanogaster

Reaction

Reaction Comment Organism Reaction ID
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Drosophila melanogaster
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Bos taurus
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Doryteuthis pealeii
ATP + H2O + a kinesin associated with a microtubule at position n = ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end) structurally almost identical to EC3.6.4.3 (microtubule-severing ATPase) but the movement it catalyses is towards the minus end of microtubules Tetrahymena sp.

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Bos taurus
-

Storage Stability

Storage Stability Organism
-80°C, 0.3 M NaCl, stored in liquid nitrogen Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2',3'-dideoxy-ATP + H2O + a kinesin associated with a microtubule at position n
-
Drosophila sp. (in: flies) 2',3'-dideoxy-ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
2'-dATP + H2O + a kinesin associated with a microtubule at position n
-
Drosophila melanogaster 2'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
2'-dATP + H2O + a kinesin associated with a microtubule at position n
-
Drosophila sp. (in: flies) 2'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
2'-dATP + H2O + a kinesin associated with a microtubule at position n
-
Doryteuthis pealeii 2'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
3'-dATP + H2O + a kinesin associated with a microtubule at position n
-
Drosophila melanogaster 3'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
3'-dATP + H2O + a kinesin associated with a microtubule at position n
-
Drosophila sp. (in: flies) 3'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
3'-dATP + H2O + a kinesin associated with a microtubule at position n
-
Doryteuthis pealeii 3'-dADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
ATP + H2O + a kinesin associated with a microtubule at position n
-
Drosophila melanogaster ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
ATP + H2O + a kinesin associated with a microtubule at position n
-
Drosophila sp. (in: flies) ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
ATP + H2O + a kinesin associated with a microtubule at position n
-
Bos taurus ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
ATP + H2O + a kinesin associated with a microtubule at position n
-
Doryteuthis pealeii ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
ATP + H2O + a kinesin associated with a microtubule at position n
-
Tetrahymena sp. ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
additional information GTP is no substrate Drosophila melanogaster ?
-
?
additional information GTP is no substrate Doryteuthis pealeii ?
-
?
N6-methyl-ATP + H2O + a kinesin associated with a microtubule at position n
-
Drosophila melanogaster N6-methyl-ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?
N6-methyl-ATP + H2O + a kinesin associated with a microtubule at position n
-
Doryteuthis pealeii N6-methyl-ADP + phosphate + a kinesin associated with a microtubule at position n-1 (toward the minus end)
-
?

Subunits

Subunits Comment Organism
dimer ncd polypeptide expressed in Escherichia coli Drosophila melanogaster

Synonyms

Synonyms Comment Organism
ncd
-
Drosophila melanogaster
ncd
-
Drosophila sp. (in: flies)
ncd
-
Doryteuthis pealeii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Drosophila sp. (in: flies)
7
-
ncd and cytoplasmic dynein Drosophila melanogaster