Cloned (Comment) | Organism |
---|---|
overexpression of the catalytic subunit Kat60 in Drosophila S2 cells | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + microtubule | Drosophila melanogaster | - |
ADP + phosphate + alpha/beta tubulin heterodimers | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9VN89 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + microtubule | - |
Drosophila melanogaster | ADP + phosphate + alpha/beta tubulin heterodimers | - |
? | |
ATP + H2O + microtubule | the microtubule-interacting and trafficking (MIT) domain and adjacent linker region of the Drosophila katanin catalytic subunit Kat60 cooperate to regulate microtubule severing in two distinct ways. First, the MIT domain and linker region of Kat60 decrease its abundance by enhancing its proteasome-dependent degradation. The Drosophila katanin regulatory subunit Kat80, which is required to stabilize Kat60 in cells, conversely reduces the proteasome-dependent degradation of Kat60. Second, the MIT domain and linker region of Kat60 augment its microtubule-disassembly activity by enhancing its association with microtubules | Drosophila melanogaster | ADP + phosphate + alpha/beta tubulin heterodimers | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Kat60 | - |
Drosophila melanogaster |
katanin | - |
Drosophila melanogaster |
Organism | Comment | Expression |
---|---|---|
Drosophila melanogaster | cells induced to express the catalytic subunit Kat60 have reduced levels of alpha-tubulin compared to control cells | up |
General Information | Comment | Organism |
---|---|---|
malfunction | cells overexpressing Kat60 display a disorganized array of fragmented microtubules and the overall length of microtubules appears to decrease with increasing levels of Kat60. Depletion of catalytic subunit Kat60 and regulatory subunit Kat80 causes minimal perturbation of steady-state alpha-tubulin levels in cells | Drosophila melanogaster |