Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli BL21 | Homo sapiens |
expression of the selenomethionine-substituted (Se-MEI-1/MEI-2) katanin complex in Escherichia coli BL21 | Caenorhabditis elegans |
Crystallization (Comment) | Organism |
---|---|
hanging-drop method at 20°C, crystal structure of KATNAL1 AAA ATPase in the nucleotide-free state reveals a monomer | Homo sapiens |
hanging-drop method at 20°C, crystal structure of MEI-1 AAA ATPase in the ADP-bound state reveals a pseudo-hexameric left-handed spiral assembly | Caenorhabditis elegans |
Protein Variants | Comment | Organism |
---|---|---|
E308Q | inactive mutant enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | in solution, wild-type katanin is in an autoinhibited state in the presence or absence of nucleotide | Caenorhabditis elegans | |
additional information | in solution, wild-type katanin is in an autoinhibited state in the presence or absence of nucleotide | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | P34808 | MEI-1 | - |
Homo sapiens | Q9BW62 | KATNAL1 | - |
Purification (Comment) | Organism |
---|---|
- |
Caenorhabditis elegans |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + H2O + microtubule | - |
Caenorhabditis elegans | ADP + phosphate + alpha/beta tubulin heterodimers | - |
? | |
ATP + H2O + microtubule | - |
Homo sapiens | ADP + phosphate + alpha/beta tubulin heterodimers | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotetramer | heterotetramer of KATNAL1/B1 (an ATPase associated with diverse cellular activities) and the regulatory subunit con80. It does not assemble beyond a dimer of heterodimers (heterotetramer), even at concentrations up to 0.05 mM | Caenorhabditis elegans |
heterotetramer | heterotetramer of KATNAL1/B1 (an ATPase associated with diverse cellular activities) and the regulatory subunit con80. It does not assemble beyond a dimer of heterodimers (heterotetramer), even at concentrations up to 0.05 mM | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
KATNAL1 | - |
Homo sapiens |
mei-1 | - |
Caenorhabditis elegans |