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Literature summary for 5.6.1.1 extracted from

  • Nithianantham, S.; McNally, F.J.; Al-Bassam, J.
    Structural basis for disassembly of katanin heterododecamers (2018), J. Biol. Chem., 293, 10590-10605 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 Homo sapiens
expression of the selenomethionine-substituted (Se-MEI-1/MEI-2) katanin complex in Escherichia coli BL21 Caenorhabditis elegans

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop method at 20°C, crystal structure of KATNAL1 AAA ATPase in the nucleotide-free state reveals a monomer Homo sapiens
hanging-drop method at 20°C, crystal structure of MEI-1 AAA ATPase in the ADP-bound state reveals a pseudo-hexameric left-handed spiral assembly Caenorhabditis elegans

Protein Variants

Protein Variants Comment Organism
E308Q inactive mutant enzyme Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information in solution, wild-type katanin is in an autoinhibited state in the presence or absence of nucleotide Caenorhabditis elegans
additional information in solution, wild-type katanin is in an autoinhibited state in the presence or absence of nucleotide Homo sapiens

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans P34808 MEI-1
-
Homo sapiens Q9BW62 KATNAL1
-

Purification (Commentary)

Purification (Comment) Organism
-
Caenorhabditis elegans
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + microtubule
-
Caenorhabditis elegans ADP + phosphate + alpha/beta tubulin heterodimers
-
?
ATP + H2O + microtubule
-
Homo sapiens ADP + phosphate + alpha/beta tubulin heterodimers
-
?

Subunits

Subunits Comment Organism
heterotetramer heterotetramer of KATNAL1/B1 (an ATPase associated with diverse cellular activities) and the regulatory subunit con80. It does not assemble beyond a dimer of heterodimers (heterotetramer), even at concentrations up to 0.05 mM Caenorhabditis elegans
heterotetramer heterotetramer of KATNAL1/B1 (an ATPase associated with diverse cellular activities) and the regulatory subunit con80. It does not assemble beyond a dimer of heterodimers (heterotetramer), even at concentrations up to 0.05 mM Homo sapiens

Synonyms

Synonyms Comment Organism
KATNAL1
-
Homo sapiens
mei-1
-
Caenorhabditis elegans