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Literature summary for 5.6.1.1 extracted from

  • Joly, N.; Martino, L.; Gigant, E.; Dumont, J.; Pintard, L.
    Microtubule-severing activity of the AAA+ ATPase Katanin is essential for female meiotic spindle assembly (2016), Development, 143, 3604-3614 .
    View publication on PubMed
Search for more literature for 5.6.1.1

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli BL21 (DE3) Caenorhabditis elegans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.45
-
 ATP pH 8.0, 28°C, MEI-1 WT/MEI-2 wild-type enzyme Caenorhabditis elegans
2.19
-
 ATP pH 8.0, 28°C, mutant enzyme MEI-1 K293A/MEI-2 Caenorhabditis elegans
2.28
-
 ATP pH 8.0, 28°C, mutant enzyme MEI-1 K293Q/MEI-2 Caenorhabditis elegans
2.94
-
 ATP pH 8.0, 28°C, mutant enzyme MEI-1 K239A/MEI-2 Caenorhabditis elegans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + microtubule Caenorhabditis elegans assembly of female meiotic spindles requires MEI-1 and MEI-2, which constitute the microtubule-severing AAA+ ATPase Katanin ADP + phosphate + alpha/beta tubulin heterodimers
-
 ?

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans P34808
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Caenorhabditis elegans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + microtubule
-
 Caenorhabditis elegans ADP + phosphate + alpha/beta tubulin heterodimers
-
 ?
ATP + H2O + microtubule assembly of female meiotic spindles requires MEI-1 and MEI-2, which constitute the microtubule-severing AAA+ ATPase Katanin Caenorhabditis elegans ADP + phosphate + alpha/beta tubulin heterodimers
-
 ?

Subunits

Subunits Comment Organism
hexamer MEI-2 subunit stabilizes the MEI-1 hexamer for optimal ATP-dependent microtubule severing Caenorhabditis elegans

Synonyms

Synonyms Comment Organism
katanin
-
 Caenorhabditis elegans

General Information

General Information Comment Organism
malfunction variants show disorganized microtubules that lack focused spindle poles reminiscent of the Katanin loss-of-function phenotype, demonstrating that the microtubule-severing activity is essential for meiotic spindle assembly in Caenorhabditis elegans Caenorhabditis elegans
physiological function assembly of female meiotic spindles requires MEI-1 and MEI-2, which constitute the microtubule-severing AAA+ ATPase Katanin Caenorhabditis elegans