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Literature summary for 5.6.1.1 extracted from
- Katanin p60 contributes to microtubule instability around the midbody and facilitates cytokinesis in rat cells (2013), PLoS ONE, 8, e80392.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Katna1, cloning of the enzyme katanin p60 subunit from rat liver, recombinant expression of His-tagged protein in Escherichia coli strain BL21(DE3) pLysS | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microtubule | katanin p60 is bundled with microtubules by contractility of the contractile ring and distributed in a microtubule-dependent manner. Katanin p60 localization is restricted in the area of non-growing microtubules during cytokinesis | Rattus norvegicus | 5874 | - |
| additional information | katanin p60 is localized to both the spindle pole and midbody during mitosis. Localized at the midzone to midbody from anaphase to cytokinesis, the enzyme shows a ring-shaped distribution in the gap between the inside of the contractile ring and the central spindle bundle in telophase. Katanin p60 does not bind with p80 at the midzone or midbody, and localization depends on microtubules. Midbody localization of katanin is dependent on central spindle structure, while Katanin p60 localization in the central spindle is not dependent on contractile ring activity | Rattus norvegicus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Rattus norvegicus |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 55000, recombinant katanin p60 subunit, SDS-PAGE | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Rattus norvegicus | - |
ADP + phosphate | - |
? | |
| additional information | Rattus norvegicus | katanin p60 destabilizes microtubules at the central spindle and the midbody | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | Q6E0V2 | katanin p60 ATPase-containing subunit A1; gene Katna1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged katanin p60 subunit from Escherichia coli strain BL21(DE3) pLysS by nickel affinity chromatography to over 95% purity | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| FAA-HTC1 cell | - |
Rattus norvegicus | - |
| hepatocellular carcinoma cell | - |
Rattus norvegicus | - |
| liver | - |
Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Rattus norvegicus | ADP + phosphate | - |
? | |
| additional information | katanin p60 destabilizes microtubules at the central spindle and the midbody | Rattus norvegicus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 55000, recombinant katanin p60 subunit, SDS-PAGE | Rattus norvegicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| katanin p60 | - |
Rattus norvegicus |
| katanin p60 ATPase-containing subunit A1 | - |
Rattus norvegicus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme is a member of a family of AAA+ ATPases (ATPase associated with various cellular activities) | Rattus norvegicus |
| malfunction | inhibition of katanin p60 results in incomplete cytokinesis by regression and thus caused the appearance of binucleate cells | Rattus norvegicus |
| physiological function | katanin is a microtubule-severing protein comprised of an AAA+ ATPase subunit and an accessory subunit designated as p60 and p80, respectively. Katanin p60 (ATPase-containing) subunit A1 does not bind with p80 at the midzone or midbody, and localization depends on microtubules. At the central spindle and the midbody, no microtubule growth plus termini are seen with katanin p60, and microtubule density is inversely correlated with katanin p60 density in the region of katanin p60 localization that seems to lead to microtubule destabilization at the midbody. Katanin p60 contributes to microtubule instability at the midzone and midbody and facilitates cytokinesis in rat cells, microtubule destabilization function of katanin p60 at the midzone and the midbody during cell division contributing to accurate cytokinesis | Rattus norvegicus |
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