Literature summary for 5.6.1.1 extracted from

Zhang, D.; Grode, K.D.; Stewman, S.F.; Diaz-Valencia, J.D.; Liebling, E.; Rath, U.; Riera, T.; Currie, J.D.; Buster, D.W.; Asenjo, A.B.; Sosa, H.J.; Ross, J.L.; Ma, A.; Rogers, S.L.; Sharp, D.J.
Drosophila katanin is a microtubule depolymerase that regulates cortical-microtubule plus-end interactions and cell migration (2011), Nat. Cell Biol., 13, 361-369.

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Cloned(Commentary)

Cloned (Comment)	Organism
baculovirus-expressed	Drosophila melanogaster

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell cortex	Dm-Kat60 concentrates at the cell cortex of S2 Drosophila cells during interphase, where it suppresses the polymerization of microtubule plus-ends	Drosophila melanogaster	5938	-
additional information	Dm-Kat60 also generally co-localizes with cortical actin. Cortical localization od Dm-Kat60 occurs indepently of microtubules, as it persists following microtubule depolymerization with colchicine	Drosophila melanogaster	-	-
additional information	Dm-Kat60 also localizes at the leading edge of migratory D17 Drosophila cells and negatively regulates multiple parameters of their motility	Drosophila melanogaster	-	-

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
baculovirus-expressed, recombinant DM-Kat60	Drosophila melanogaster

Reaction

Reaction	Comment	Organism	Reaction ID
n ATP + n H2O + a microtubule = n ADP + n phosphate + (n+1) alpha/beta tubulin heterodimers	Dm-Kat60 severs and depolymerizes microtubules from their ends	Drosophila melanogaster	a

Source Tissue

Source Tissue	Comment	Organism	Textmining
Drosophila D17 cell	-	Drosophila melanogaster	-
SCHNEIDER-2 cell	S2 cell	Drosophila melanogaster	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + H2O	recombinant Dm-Kat60 robustly severs taxol-stabilized microtubules in the presence of ATP. This reacton is inhibited when ATP is depleted with hexokinase and glucose or replaced with the non-hydrolysable ATP analogue 5'-adenylyl-beta-gamma-imidotriphosphate AMPPMP	Drosophila melanogaster	ADP + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
Dm-Kat60	-	Drosophila melanogaster
Drosophila katanin Dm-Kat60	-	Drosophila melanogaster
katanin	-	Drosophila melanogaster

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Release 2023.1 (January 2023)