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Literature summary for 5.6.1.1 extracted from
- Arabidopsis katanin binds microtubules using a multimeric microtubule-binding domain (2007), Plant Physiol. Biochem., 45, 867-877.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| microtubule | - |
Arabidopsis thaliana |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| three domains of AtKSS are defined, AtKSS1, aa 1-34, AtKSS2, aa 35-210, AtKSS3, the ATPase domain, aa 211-523, in addition, combinations are defined, AtKKS12 and AtKKS23, AtKSS1,2,3,12,and 23 are cloned into pGEX-4T-1 and pOL-LT plasmids | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
GST-AtKSS1, determined by SDS-PAGE | Arabidopsis thaliana |
| 46000 | - |
GST-AtKSS2, determined by SDS-PAGE | Arabidopsis thaliana |
| 50000 | - |
GST-AtKSS12, determined by SDS-PAGE | Arabidopsis thaliana |
| 60000 | - |
catalytic subunit p60 | Arabidopsis thaliana |
| 62000 | - |
GST-AtKSS3, determined by SDS-PAGE | Arabidopsis thaliana |
| 66000 | - |
GST-AtKSS13, determined by SDS-PAGE | Arabidopsis thaliana |
| 80000 | - |
regulatory subunit p80 | Arabidopsis thaliana |
| 82000 | - |
GST-AtKSS23, determined by SDS-PAGE | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | Arabidopsis thaliana | - |
ADP + phosphate | - |
ir |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q9SEX2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using a glutathione Sepharose 4B column | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | - |
Arabidopsis thaliana | ADP + phosphate | - |
ir | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | katanin is a heterodimer composed of a catalytic subunit that hydrolyses ATP to sever microtubules, and a regulatory subunit involved in subcellular targeting | Arabidopsis thaliana |
| More | data suggest that AtKSS oligomerizes into a ring-shaped oligomer, and that the microtubule-binding domain is involved in the oligomerization process, the addition of two trimers to form a hexamer is proposed | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtKSS | katanin catalytic subunit, microtubule-stimulated ATPase activity | Arabidopsis thaliana |
| katanin | - |
Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.9 | - |
ATPase activity assay | Arabidopsis thaliana |
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